Satellite cells isolated from skeletal muscle of adult mice undergo a process of differentiation in culture. Treatment of dissociated muscle fragments with collagenase, followed by three sequential treatments with trypsin, results in a cell suspension highly enriched in myogenic cells. This suspension is plated in collagen coated dishes and cultured in MEM supplemented with 10% horse serum and 3% embryo extract. Cell population in culture is mainly composed of myogenic cells which duplicate actively during the first two days of culture, then withdraw from the cell cycle and fuse to form multinucleated myotubes, with a concomitant increase of creatine phosphokinase (CPK) activity. © Taylor & Francis Group, LLC.
Differentiation in culture of myogenic cells from adult mouse muscle / Cossu, G.; Bouche, M.; Zani, B.; Molinaro, M.. - In: BOLLETTINO DI ZOOLOGIA. - ISSN 0373-4137. - 45:4(1978), pp. 369-374. [10.1080/11250007809440145]
Differentiation in culture of myogenic cells from adult mouse muscle
Cossu G.;Bouche M.;Zani B.;Molinaro M.
1978
Abstract
Satellite cells isolated from skeletal muscle of adult mice undergo a process of differentiation in culture. Treatment of dissociated muscle fragments with collagenase, followed by three sequential treatments with trypsin, results in a cell suspension highly enriched in myogenic cells. This suspension is plated in collagen coated dishes and cultured in MEM supplemented with 10% horse serum and 3% embryo extract. Cell population in culture is mainly composed of myogenic cells which duplicate actively during the first two days of culture, then withdraw from the cell cycle and fuse to form multinucleated myotubes, with a concomitant increase of creatine phosphokinase (CPK) activity. © Taylor & Francis Group, LLC.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
