The aim of present study was to develop radiolabeled NPs to overcome the limitations of fluorescence with theranostic potential. Synthesis of PLGA-NPs loaded with technetium-99m was based on a Dean-Vortex-Bifurcation Mixer (DVBM) using an innovative microfluidic technique with high batch-to-batch reproducibility and tailored-made size of NPs. Eighteen different formulations were tested and characterized for particle size, zeta potential, polydispersity index, labeling efficiency, and in vitro stability. Overall, physical characterization by dynamic light scattering (DLS) showed an increase in particle size after radiolabeling probably due to the incorporation of the isotope into the PLGA-NPs shell. NPs of 60 nm (obtained by 5:1 PVA:PLGA ratio and 15 mL/min TFR with99mTc included in PVA) had high labeling efficiency (94.20 ± 5.83%) and > 80% stability after 24 h and showed optimal biodistribution in BALB/c mice. In conclusion, we confirmed the possibility of radiolabeling NPs with99mTc using the microfluidics and provide best formulation for tumor targeting studies.

Synthesis and biodistribution of99mTc-labeled PLGA nanoparticles by microfluidic technique / Varani, M.; Campagna, G.; Bentivoglio, V.; Serafinelli, M.; Martini, M. L.; Galli, F.; Signore, A.. - In: PHARMACEUTICS. - ISSN 1999-4923. - 13:11(2021). [10.3390/pharmaceutics13111769]

Synthesis and biodistribution of99mTc-labeled PLGA nanoparticles by microfluidic technique

Varani M.
;
Campagna G.;Galli F.;Signore A.
2021

Abstract

The aim of present study was to develop radiolabeled NPs to overcome the limitations of fluorescence with theranostic potential. Synthesis of PLGA-NPs loaded with technetium-99m was based on a Dean-Vortex-Bifurcation Mixer (DVBM) using an innovative microfluidic technique with high batch-to-batch reproducibility and tailored-made size of NPs. Eighteen different formulations were tested and characterized for particle size, zeta potential, polydispersity index, labeling efficiency, and in vitro stability. Overall, physical characterization by dynamic light scattering (DLS) showed an increase in particle size after radiolabeling probably due to the incorporation of the isotope into the PLGA-NPs shell. NPs of 60 nm (obtained by 5:1 PVA:PLGA ratio and 15 mL/min TFR with99mTc included in PVA) had high labeling efficiency (94.20 ± 5.83%) and > 80% stability after 24 h and showed optimal biodistribution in BALB/c mice. In conclusion, we confirmed the possibility of radiolabeling NPs with99mTc using the microfluidics and provide best formulation for tumor targeting studies.
2021
microfluidics; nuclear medicine; poly (lactic-co-glycolic acid) (PLGA); radiolabeled nanoparticles
01 Pubblicazione su rivista::01a Articolo in rivista
Synthesis and biodistribution of99mTc-labeled PLGA nanoparticles by microfluidic technique / Varani, M.; Campagna, G.; Bentivoglio, V.; Serafinelli, M.; Martini, M. L.; Galli, F.; Signore, A.. - In: PHARMACEUTICS. - ISSN 1999-4923. - 13:11(2021). [10.3390/pharmaceutics13111769]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1589323
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