The aim of the present study was to investigate the possible protective effects of a garlic hydroalcoholic extract on the burden of oxidative stress and inflammation occurring on mouse heart specimens exposed to E. coli lipopolysaccharide (LPS), which is a well-established inflammatory stimulus. Headspace solid-phase microextraction combined with the gas chromatography–mass spectrometry (HS-SPME/GC–MS) technique was applied to determine the volatile fraction of the garlic powder, and the HS-SPME conditions were optimized for each of the most representative classes of compounds. CIEL*a*b* colorimetric analyses were performed on the powder sample at the time of delivery, after four and after eight months of storage at room temperature in the dark, to evaluate the color changing. Freshly prepared hydroalcoholic extract was also evaluated in its color character. Furthermore, the hydroalcoholic extract was analyzed through GC–MS. The extract was found to be able to significantly inhibit LPS-induced prostaglandin (PG) E2 and 8-iso-PGF2α levels, as well as mRNA levels of cyclooxygenase (COX)-2, interleukin (IL)-6, and nuclear factor-kB (NF-kB), in heart specimens. Concluding, our findings showed that the garlic hydroalcoholic extract exhibited cardioprotective effects on multiple inflammatory and oxidative stress pathways.

Protective effects induced by a hydroalcoholic allium sativum extract in isolated mouse heart / Recinella, L.; Chiavaroli, A.; Masciulli, F.; Fraschetti, C.; Filippi, A.; Cesa, S.; Cairone, F.; Gorica, E.; De Leo, M.; Braca, A.; Martelli, A.; Calderone, V.; Orlando, G.; Ferrante, C.; Menghini, L.; Di Simone, S. C.; Veschi, S.; Cama, A.; Brunetti, L.; Leone, S.. - In: NUTRIENTS. - ISSN 2072-6643. - 13:7(2021), pp. 1-21. [10.3390/nu13072332]

Protective effects induced by a hydroalcoholic allium sativum extract in isolated mouse heart

Masciulli F.;Fraschetti C.;Filippi A.;Cesa S.;Cairone F.;De Leo M.;Braca A.;
2021

Abstract

The aim of the present study was to investigate the possible protective effects of a garlic hydroalcoholic extract on the burden of oxidative stress and inflammation occurring on mouse heart specimens exposed to E. coli lipopolysaccharide (LPS), which is a well-established inflammatory stimulus. Headspace solid-phase microextraction combined with the gas chromatography–mass spectrometry (HS-SPME/GC–MS) technique was applied to determine the volatile fraction of the garlic powder, and the HS-SPME conditions were optimized for each of the most representative classes of compounds. CIEL*a*b* colorimetric analyses were performed on the powder sample at the time of delivery, after four and after eight months of storage at room temperature in the dark, to evaluate the color changing. Freshly prepared hydroalcoholic extract was also evaluated in its color character. Furthermore, the hydroalcoholic extract was analyzed through GC–MS. The extract was found to be able to significantly inhibit LPS-induced prostaglandin (PG) E2 and 8-iso-PGF2α levels, as well as mRNA levels of cyclooxygenase (COX)-2, interleukin (IL)-6, and nuclear factor-kB (NF-kB), in heart specimens. Concluding, our findings showed that the garlic hydroalcoholic extract exhibited cardioprotective effects on multiple inflammatory and oxidative stress pathways.
2021
Bioinformatics; CIEL*a*b*; Garlic; HS-SPME/GC–MS; inflammation; multimethodological evaluation; oxidative stress; animals; antioxidants; cardiotonic agents; garlic; gas chromatography-mass spectrometry; heart; mice; oxidative stress; plant extracts; solid phase microextraction
01 Pubblicazione su rivista::01a Articolo in rivista
Protective effects induced by a hydroalcoholic allium sativum extract in isolated mouse heart / Recinella, L.; Chiavaroli, A.; Masciulli, F.; Fraschetti, C.; Filippi, A.; Cesa, S.; Cairone, F.; Gorica, E.; De Leo, M.; Braca, A.; Martelli, A.; Calderone, V.; Orlando, G.; Ferrante, C.; Menghini, L.; Di Simone, S. C.; Veschi, S.; Cama, A.; Brunetti, L.; Leone, S.. - In: NUTRIENTS. - ISSN 2072-6643. - 13:7(2021), pp. 1-21. [10.3390/nu13072332]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1573028
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