The tumor-associated isoenzymes hCA IX and hCA XII catalyze the hydration of carbon dioxide to bicarbonate and protons. These isoforms are highly overexpressed in many types of cancer, where they contribute to the acidification of the tumor environment, promoting tumor cell invasion and metastasis. In this work, in order to identify novel dual hCA IX and XII inhibitors, virtual screening techniques and biological assays were combined. A structure-based virtual screening towards hCA IX and XII was performed using a database of approximately 26,000 natural compounds. The best shared hits were submitted to a thermodynamic analysis and three promising best hits were identified and evaluated in terms of their hCA IX and XII inhibitor activity. In vitro biological assays were in line with the theoretical studies and revealed that syringin, lithospermic acid, and (-)-dehydrodiconiferyl alcohol behave as good hCA IX and hCA XII dual inhibitors.

In silico identification and biological evaluation of antioxidant food components endowed with IX and XII hCA inhibition / Costa, G.; Maruca, A.; Rocca, R.; Ambrosio, F. A.; Berrino, E.; Carta, F.; Mesiti, F.; Salatino, A.; Lanzillotta, D.; Trapasso, F.; Artese, A.; Alcaro, S.; Supuran, C. T.. - In: ANTIOXIDANTS. - ISSN 2076-3921. - 9:(2020), pp. 1-13. [10.3390/antiox9090775]

In silico identification and biological evaluation of antioxidant food components endowed with IX and XII hCA inhibition

Berrino E.;Supuran C. T.
2020

Abstract

The tumor-associated isoenzymes hCA IX and hCA XII catalyze the hydration of carbon dioxide to bicarbonate and protons. These isoforms are highly overexpressed in many types of cancer, where they contribute to the acidification of the tumor environment, promoting tumor cell invasion and metastasis. In this work, in order to identify novel dual hCA IX and XII inhibitors, virtual screening techniques and biological assays were combined. A structure-based virtual screening towards hCA IX and XII was performed using a database of approximately 26,000 natural compounds. The best shared hits were submitted to a thermodynamic analysis and three promising best hits were identified and evaluated in terms of their hCA IX and XII inhibitor activity. In vitro biological assays were in line with the theoretical studies and revealed that syringin, lithospermic acid, and (-)-dehydrodiconiferyl alcohol behave as good hCA IX and hCA XII dual inhibitors.
2020
Dual inhibitors; HCA IX and XII; In vitro assays; Molecular modeling studies
01 Pubblicazione su rivista::01a Articolo in rivista
In silico identification and biological evaluation of antioxidant food components endowed with IX and XII hCA inhibition / Costa, G.; Maruca, A.; Rocca, R.; Ambrosio, F. A.; Berrino, E.; Carta, F.; Mesiti, F.; Salatino, A.; Lanzillotta, D.; Trapasso, F.; Artese, A.; Alcaro, S.; Supuran, C. T.. - In: ANTIOXIDANTS. - ISSN 2076-3921. - 9:(2020), pp. 1-13. [10.3390/antiox9090775]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1555679
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