To investigate the effects of oral bacteriotherapy on intestinal phenylalanine and tyrosine metabolism, in this longitudinal, double-arm trial, 15 virally suppressed HIV+ individuals underwent blood and fecal sample collection at baseline and after 6 months of oral bacteriotherapy. A baseline fecal sample was collected from 15 healthy individuals and served as control group for the baseline levels of fecal phenylalanine and tyrosine. CD4 and CD8 immune activation (CD38+) was evaluated by flow cytometry. Amino acid evaluation on fecal samples was conducted by Proton Nuclear Magnetic Resonance. Results showed that HIV+ participants displayed higher baseline phenylalanine/tyrosine ratio values than healthy volunteers. A significand reduction in phenylalanine/tyrosine ratio and peripheral CD4+ CD38+ activation was observed at the end of oral bacteriotherapy. In conclusion, probiotics beneficially affect the immune activation of HIV+ individuals. Therefore, the restoration of intestinal amino acid metabolism could represent the mechanisms through which probiotics exert these desirable effects.
Modulation of phenylalanine and tyrosine metabolism in HIV-1 infected patients with neurocognitive impairment: results from a clinical trial / Innocenti, Giuseppe P.; Santinelli, Letizia; Laghi, Luca; Borrazzo, Cristian; Pinacchio, Claudia; Fratino, Mariangela; Celani, Luigi; Cavallari, Eugenio N.; Scagnolari, Carolina; Frasca, Federica; Antonelli, Guido; Mastroianni, Claudio M.; D’Ettorre, Gabriella; Ceccarelli, Giancarlo. - In: METABOLITES. - ISSN 2218-1989. - 10:7(2020). [10.3390/metabo10070274]
Modulation of phenylalanine and tyrosine metabolism in HIV-1 infected patients with neurocognitive impairment: results from a clinical trial
Innocenti, Giuseppe P.Primo
Conceptualization
;Santinelli, Letizia
Secondo
Conceptualization
;Borrazzo, CristianData Curation
;Pinacchio, ClaudiaData Curation
;Fratino, MariangelaValidation
;Celani, LuigiInvestigation
;Cavallari, Eugenio N.Investigation
;Scagnolari, CarolinaProject Administration
;Frasca, FedericaData Curation
;Antonelli, GuidoWriting – Review & Editing
;Mastroianni, Claudio M.Supervision
;d’Ettorre, GabriellaPenultimo
Writing – Review & Editing
;Ceccarelli, GiancarloUltimo
Writing – Review & Editing
2020
Abstract
To investigate the effects of oral bacteriotherapy on intestinal phenylalanine and tyrosine metabolism, in this longitudinal, double-arm trial, 15 virally suppressed HIV+ individuals underwent blood and fecal sample collection at baseline and after 6 months of oral bacteriotherapy. A baseline fecal sample was collected from 15 healthy individuals and served as control group for the baseline levels of fecal phenylalanine and tyrosine. CD4 and CD8 immune activation (CD38+) was evaluated by flow cytometry. Amino acid evaluation on fecal samples was conducted by Proton Nuclear Magnetic Resonance. Results showed that HIV+ participants displayed higher baseline phenylalanine/tyrosine ratio values than healthy volunteers. A significand reduction in phenylalanine/tyrosine ratio and peripheral CD4+ CD38+ activation was observed at the end of oral bacteriotherapy. In conclusion, probiotics beneficially affect the immune activation of HIV+ individuals. Therefore, the restoration of intestinal amino acid metabolism could represent the mechanisms through which probiotics exert these desirable effects.File | Dimensione | Formato | |
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Innocenti_Modulation of Phenylalanine_2020.pdf
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Note: https://www.mdpi.com/2218-1989/10/7/274
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