Aim: The aim of this study was to analyze, by the aid of microbiological analysis and the field emission scanning electron microscopical (FESEM) analysis, the role of high-density polytetrafluoroethylene (d-PTFE) membranes in avoiding the microbial colonization of a nanocrystalline hydroxyapatite (nc-HA) bone graft and the involvement of this colonization in the healing process. Materials and methods: Six patients underwent extraction of unrecoverable teeth, and a socket preservation technique was carried out with nc-HA synthetic bone graft and then covered with a d-PTFE membrane. After 28 days from surgery, FE-SEM analysis and BioTimer assay technique to assess the microbiological count of streptococci species were carried out. Data were collected and analyzed by the Student's t test (confidence interval: 95%). Results: The mean amount of bacteria measured on the upper side of the membrane was 6.52 ± 0.50 CFU, while on the lower side, it was 6.59 ± 0.40 CFU. Significant differences were not found between the two sides of the membrane or between the different sectors (p > 0.05). The FE-SEM analysis revealed structured biofilms on both sides of the membrane: species of cocci, bacilli, and fusobacteria were recognizable in occasional settled vegetations. Conclusion: Since the amount of bacteria found was low, the improved impermeability of the d-PTFE membrane permitted the healing process to proceed uneventful and without signs of infection or inflammation. Clinical relevance: The infection of the graft site could lead to a failure of the socket preservation technique which could delay or compromise the rehabilitation following procedures. The use of d-PTFE can improve the bone regeneration thanks to its antimicrobial properties.

Microbiological and FE-SEM assessment of d-PTFE membrane exposed to oral environment after alveolar socket preservation managed with granular nc-HA / Mazzucchi, G.; Lollobrigida, M.; Laurito, D.; di Nardo, D.; Berlutti, F.; Passariello, C.; Serafini, G.; Testarelli, L.; de Biase, A.. - In: JOURNAL OF CONTEMPORARY DENTAL PRACTICE. - ISSN 1526-3711. - 21:4(2020), pp. 404-409. [10.5005/JP-JOURNALS-10024-2805]

Microbiological and FE-SEM assessment of d-PTFE membrane exposed to oral environment after alveolar socket preservation managed with granular nc-HA

Lollobrigida M.
Secondo
Writing – Review & Editing
;
di Nardo D.
Data Curation
;
Berlutti F.
Methodology
;
Passariello C.
Data Curation
;
Serafini G.
Writing – Review & Editing
;
Testarelli L.
Penultimo
Conceptualization
;
de Biase A.
Ultimo
Methodology
2020

Abstract

Aim: The aim of this study was to analyze, by the aid of microbiological analysis and the field emission scanning electron microscopical (FESEM) analysis, the role of high-density polytetrafluoroethylene (d-PTFE) membranes in avoiding the microbial colonization of a nanocrystalline hydroxyapatite (nc-HA) bone graft and the involvement of this colonization in the healing process. Materials and methods: Six patients underwent extraction of unrecoverable teeth, and a socket preservation technique was carried out with nc-HA synthetic bone graft and then covered with a d-PTFE membrane. After 28 days from surgery, FE-SEM analysis and BioTimer assay technique to assess the microbiological count of streptococci species were carried out. Data were collected and analyzed by the Student's t test (confidence interval: 95%). Results: The mean amount of bacteria measured on the upper side of the membrane was 6.52 ± 0.50 CFU, while on the lower side, it was 6.59 ± 0.40 CFU. Significant differences were not found between the two sides of the membrane or between the different sectors (p > 0.05). The FE-SEM analysis revealed structured biofilms on both sides of the membrane: species of cocci, bacilli, and fusobacteria were recognizable in occasional settled vegetations. Conclusion: Since the amount of bacteria found was low, the improved impermeability of the d-PTFE membrane permitted the healing process to proceed uneventful and without signs of infection or inflammation. Clinical relevance: The infection of the graft site could lead to a failure of the socket preservation technique which could delay or compromise the rehabilitation following procedures. The use of d-PTFE can improve the bone regeneration thanks to its antimicrobial properties.
2020
Alveolar socket preservation; Bacterial contamination; Bone graft; Dense polytetrafluoroethylene; Nanocrystalline hydroxyapatite
01 Pubblicazione su rivista::01a Articolo in rivista
Microbiological and FE-SEM assessment of d-PTFE membrane exposed to oral environment after alveolar socket preservation managed with granular nc-HA / Mazzucchi, G.; Lollobrigida, M.; Laurito, D.; di Nardo, D.; Berlutti, F.; Passariello, C.; Serafini, G.; Testarelli, L.; de Biase, A.. - In: JOURNAL OF CONTEMPORARY DENTAL PRACTICE. - ISSN 1526-3711. - 21:4(2020), pp. 404-409. [10.5005/JP-JOURNALS-10024-2805]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1420090
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