We present here a simple method for the rapid quantification of squalene in vegetable oils by NMR (Nuclear Magnetic Resonance). The method was validated by adding internal standards to several vegetable oil samples. Quantification was accomplished by exploiting the characteristic resolved signal of terminal methyl groups at 1.67 ppm, which allowed squalene quantification regardless of the hydrophobic matrix. Theoretical principles are fulfilled by the method and, despite the general belief that NMR displays intrinsic low sensitivity, acceptable accuracy (<4%) and reproducibility (<6%) can be reached when squalene is over 2000 ppm, even in “worst case scenarios”. This method may be useful in the continuing efforts to rapidly generate accurate and complete quantitative data suitable for inclusion in the identification labels of vegetable oil products.
Quick unreferenced NMR quantification of Squalene in vegetable oils / Rotondo, Archimede; Salvo, Andrea; Gallo, Vito; Rastrelli, Luca; Dugo, Giacomo. - In: EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY. - ISSN 1438-7697. - 119:11(2017), pp. 1-9. [10.1002/ejlt.201700151]
Quick unreferenced NMR quantification of Squalene in vegetable oils
SALVO, Andrea;
2017
Abstract
We present here a simple method for the rapid quantification of squalene in vegetable oils by NMR (Nuclear Magnetic Resonance). The method was validated by adding internal standards to several vegetable oil samples. Quantification was accomplished by exploiting the characteristic resolved signal of terminal methyl groups at 1.67 ppm, which allowed squalene quantification regardless of the hydrophobic matrix. Theoretical principles are fulfilled by the method and, despite the general belief that NMR displays intrinsic low sensitivity, acceptable accuracy (<4%) and reproducibility (<6%) can be reached when squalene is over 2000 ppm, even in “worst case scenarios”. This method may be useful in the continuing efforts to rapidly generate accurate and complete quantitative data suitable for inclusion in the identification labels of vegetable oil products.File | Dimensione | Formato | |
---|---|---|---|
Rotondo_Squalene_2017.pdf
Open Access dal 01/12/2018
Tipologia:
Documento in Post-print (versione successiva alla peer review e accettata per la pubblicazione)
Licenza:
Tutti i diritti riservati (All rights reserved)
Dimensione
584.91 kB
Formato
Adobe PDF
|
584.91 kB | Adobe PDF |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.