A fast reversed-phase UPLC method was developed for squalene determination in Sicilian pistachio samples that entry in the European register of the products with P.D.O. In the present study the SPE procedure was optimized for the squalene extraction prior to the UPLC/PDA analysis. The precision of the full analytical procedure was satisfactory and the mean recoveries were 92.8 ± 0.3 % and 96.6 ± 0.1 % for 25 and 50 mg L-1 level of addition, respectively. Selected chromatographic conditions allowed a very fast squalene determination; in fact it was well separated in ∼ 0.54 min with good resolution. Squalene was detected in all the pistachio samples analyzed and the levels ranged from 55.45 to 226.34 mg . kg-1. Comparing our results with those of other studies it emerges that squalene contents in P.D.O. Sicilian pistachio samples, generally, were higher than those measured for other samples of different geographic origins.
Fast UPLC/PDA determination of squalene in Sicilian P.D.O. pistachio from Bronte: optimization of oil extraction method and analytical characterization / SALVO, Andrea; LA TORRE, Giovanna Loredana; Di Stefano, Vita; Capocchiano, Valentina; MANGANO, VALENTINA; SAIJA, EMANUELE; Pellizzeri, Vito; Casale, Katia Erminia; DUGO, Giacomo. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - 221:(2017), pp. 1631-1636. [10.1016/j.foodchem.2016.10.126]
Fast UPLC/PDA determination of squalene in Sicilian P.D.O. pistachio from Bronte: optimization of oil extraction method and analytical characterization
SALVO, Andrea;
2017
Abstract
A fast reversed-phase UPLC method was developed for squalene determination in Sicilian pistachio samples that entry in the European register of the products with P.D.O. In the present study the SPE procedure was optimized for the squalene extraction prior to the UPLC/PDA analysis. The precision of the full analytical procedure was satisfactory and the mean recoveries were 92.8 ± 0.3 % and 96.6 ± 0.1 % for 25 and 50 mg L-1 level of addition, respectively. Selected chromatographic conditions allowed a very fast squalene determination; in fact it was well separated in ∼ 0.54 min with good resolution. Squalene was detected in all the pistachio samples analyzed and the levels ranged from 55.45 to 226.34 mg . kg-1. Comparing our results with those of other studies it emerges that squalene contents in P.D.O. Sicilian pistachio samples, generally, were higher than those measured for other samples of different geographic origins.| File | Dimensione | Formato | |
|---|---|---|---|
|
Salvo_postprint_squalene_2017.pdf
accesso aperto
Tipologia:
Documento in Post-print (versione successiva alla peer review e accettata per la pubblicazione)
Licenza:
Creative commons
Dimensione
597.91 kB
Formato
Adobe PDF
|
597.91 kB | Adobe PDF | |
|
Salvo_squalene_2017.pdf
solo gestori archivio
Tipologia:
Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza:
Tutti i diritti riservati (All rights reserved)
Dimensione
397.96 kB
Formato
Adobe PDF
|
397.96 kB | Adobe PDF | Contatta l'autore |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
