Intrinsically Disordered Proteins (IDPs) are a class of protein that exert their function despite lacking a well-defined three-dimensional structure, which is sometimes achieved only upon binding to their natural ligands. This feature implies the folding of IDPs to be generally coupled with a binding event, representing an interesting challenge for kinetic studies. In this review, we recapitulate some of the most important findings of IDPs binding-induced folding mechanisms obtained by analyzing their binding kinetics. Furthermore, by focusing on the interaction between the Measles virus NTAIL protein, a prototypical IDP, and its physiological partner, the X domain, we recapitulate the major theoretical and experimental approaches that were used to describe binding induced folding.

Binding induced folding: Lessons from the kinetics of interaction between NTAIL and XD / Toto, A.; Troilo, F.; Visconti, L.; Malagrinò, F.; Bignon, C.; Longhi, Sonia Rachele Agata; Gianni, S.; S,. - In: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. - ISSN 0003-9861. - (2019), pp. 255-261. [10.1016/j.abb.2019.07.011]

Binding induced folding: Lessons from the kinetics of interaction between NTAIL and XD

Toto A.;Troilo F.;Visconti L.;Malagrinò F.;Longhi;S. Gianni;
2019

Abstract

Intrinsically Disordered Proteins (IDPs) are a class of protein that exert their function despite lacking a well-defined three-dimensional structure, which is sometimes achieved only upon binding to their natural ligands. This feature implies the folding of IDPs to be generally coupled with a binding event, representing an interesting challenge for kinetic studies. In this review, we recapitulate some of the most important findings of IDPs binding-induced folding mechanisms obtained by analyzing their binding kinetics. Furthermore, by focusing on the interaction between the Measles virus NTAIL protein, a prototypical IDP, and its physiological partner, the X domain, we recapitulate the major theoretical and experimental approaches that were used to describe binding induced folding.
2019
proteins; proteome; protein disorder
01 Pubblicazione su rivista::01a Articolo in rivista
Binding induced folding: Lessons from the kinetics of interaction between NTAIL and XD / Toto, A.; Troilo, F.; Visconti, L.; Malagrinò, F.; Bignon, C.; Longhi, Sonia Rachele Agata; Gianni, S.; S,. - In: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. - ISSN 0003-9861. - (2019), pp. 255-261. [10.1016/j.abb.2019.07.011]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1336088
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