In non-small cell lung cancer (NSCLC), there is a consensus regarding the use of liquid biopsy, generally, to detect "druggable" mutations and, in particular, to monitor tyrosine kinase inhibitor (TKI) treatments. However, whether circulating tumor cells (CTCs) are better tools than cell-free DNA (cfDNA), is still a matter of debate, mainly concerning which antigen(s) we should use to investigating simultaneously both epithelial and epithelial-to-mesenchymal transient (EMT) phenotype in the same sample of CTCs. To address this item, we exploited here a single-tube liquid biopsy, to detect both epithelial cell adhesion molecule (EpCAM)-positive CTCs and EpCAM-low/negative CTCs, because down-modulation of EpCAM is considered the first step in EMT. Furthermore, we analyzed the DNA from CTCs of four different phenotypes (ctcDNA), according to their EpCAM expression and cytokeratin pattern, and circulating tumor DNA (ctDNA) by droplet digital PCR (ddPCR), in order to disclose activating and resistancedriving mutations. Liquid biopsy reflected spatial and temporal heterogeneity of the tumor under treatment pressure. We provide the proof-of-concept that the complementary use of ctDNA and ctcDNA represents a reliable, minimally invasive and dynamic tool for a more comprehensive view of tumor evolution.

What information could the main actors of liquid biopsy provide? A representative case of non-small cell lung cancer (NSCLC) / Pezzuto, A.; Manicone, M.; Scaini, M. C.; Ricci, A.; Mariotta, S.; Zamarchi, R.; Rossi, Elisabetta. - In: JOURNAL OF THORACIC DISEASE. - ISSN 2072-1439. - ELETTRONICO. - 10:7(2018), pp. 570-576. [10.21037/jtd.2018.06.38]

What information could the main actors of liquid biopsy provide? A representative case of non-small cell lung cancer (NSCLC)

Ricci A.;Mariotta S.;Rossi, Elisabetta
2018

Abstract

In non-small cell lung cancer (NSCLC), there is a consensus regarding the use of liquid biopsy, generally, to detect "druggable" mutations and, in particular, to monitor tyrosine kinase inhibitor (TKI) treatments. However, whether circulating tumor cells (CTCs) are better tools than cell-free DNA (cfDNA), is still a matter of debate, mainly concerning which antigen(s) we should use to investigating simultaneously both epithelial and epithelial-to-mesenchymal transient (EMT) phenotype in the same sample of CTCs. To address this item, we exploited here a single-tube liquid biopsy, to detect both epithelial cell adhesion molecule (EpCAM)-positive CTCs and EpCAM-low/negative CTCs, because down-modulation of EpCAM is considered the first step in EMT. Furthermore, we analyzed the DNA from CTCs of four different phenotypes (ctcDNA), according to their EpCAM expression and cytokeratin pattern, and circulating tumor DNA (ctDNA) by droplet digital PCR (ddPCR), in order to disclose activating and resistancedriving mutations. Liquid biopsy reflected spatial and temporal heterogeneity of the tumor under treatment pressure. We provide the proof-of-concept that the complementary use of ctDNA and ctcDNA represents a reliable, minimally invasive and dynamic tool for a more comprehensive view of tumor evolution.
2018
circulating tumor cells (ctcs); circulating tumor dna (ctdna); epidermal growth factor receptor (egfr); epithelial cell adhesion molecule-positive and epithelial cell adhesion molecule-low/negative (epcam pos and epcam low/neg); liquid biopsy; non-small cell lung cancer (nsclc)
01 Pubblicazione su rivista::01i Case report
What information could the main actors of liquid biopsy provide? A representative case of non-small cell lung cancer (NSCLC) / Pezzuto, A.; Manicone, M.; Scaini, M. C.; Ricci, A.; Mariotta, S.; Zamarchi, R.; Rossi, Elisabetta. - In: JOURNAL OF THORACIC DISEASE. - ISSN 2072-1439. - ELETTRONICO. - 10:7(2018), pp. 570-576. [10.21037/jtd.2018.06.38]
File allegati a questo prodotto
File Dimensione Formato  
Pezzuto_What-information_2018.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.49 MB
Formato Adobe PDF
1.49 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1311088
Citazioni
  • ???jsp.display-item.citation.pmc??? 6
  • Scopus 10
  • ???jsp.display-item.citation.isi??? 9
social impact