Background/aim: The identification of novel prognostic biomarkers for melanoma metastasis is essential to improve patient outcomes. To this aim, we characterized miRNA expression profiles in relation to metastasis in melanoma and correlated miRNAs expression with clinical-pathological factors. Materials and methods: MiR-145-5p, miR-150-5p, miR-182-5p, miR-203-3p, miR-205-5p and miR-211-5p expression levels were analyzed in primary cutaneous melanomas, including thin and thick melanomas, and in melanoma metastases by quantitative Real-Time PCR. Results: A significantly lower miR-205-5p expression was found in metastases compared to primary melanomas. Furthermore, a progressive down-regulation of miR-205-5p expression was observed from loco-regional to distant metastasis. Significantly lower miR-145-5p and miR-203-3p expression levels were found in cases with Breslow thickness >1 mm, high Clark level, ulceration and mitotic rate ≥1/mm2 Conclusion: Our findings point to miR-205-5p as potential biomarker of distant metastases and to miR-145-5p and miR-203-3p as markers of aggressiveness in melanoma.

MiRNAs as potential prognostic biomarkers for metastasis in thin and thick primary cutaneous melanomas / Valentini, Virginia; Zelli, Veronica; Gaggiano, Emanuela; Silvestri, Valentina; Rizzolo, Piera; Bucalo, Agostino; Calvieri, Stefano; Grassi, Sara; Frascione, Pasquale; Pietro, Donati; Soda, Giuseppe; Ottini, Laura; Richetta, Antonio Giovanni. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - 39:8(2019), pp. 4085-4093. [10.21873/anticanres.13566]

MiRNAs as potential prognostic biomarkers for metastasis in thin and thick primary cutaneous melanomas

VIRGINIA, VALENTINI
Primo
;
VERONICA, ZELLI
Secondo
;
GAGGIANO, EMANUELA;VALENTINA, SILVESTRI;PIERA, RIZZOLO;AGOSTINO, BUCALO;STEFANO, CALVIERI;SARA, GRASSI;PASQUALE, FRASCIONE;GIUSEPPE, SODA;LAURA, OTTINI
Penultimo
;
ANTONIO GIOVANNI, RICHETTA
Ultimo
2019

Abstract

Background/aim: The identification of novel prognostic biomarkers for melanoma metastasis is essential to improve patient outcomes. To this aim, we characterized miRNA expression profiles in relation to metastasis in melanoma and correlated miRNAs expression with clinical-pathological factors. Materials and methods: MiR-145-5p, miR-150-5p, miR-182-5p, miR-203-3p, miR-205-5p and miR-211-5p expression levels were analyzed in primary cutaneous melanomas, including thin and thick melanomas, and in melanoma metastases by quantitative Real-Time PCR. Results: A significantly lower miR-205-5p expression was found in metastases compared to primary melanomas. Furthermore, a progressive down-regulation of miR-205-5p expression was observed from loco-regional to distant metastasis. Significantly lower miR-145-5p and miR-203-3p expression levels were found in cases with Breslow thickness >1 mm, high Clark level, ulceration and mitotic rate ≥1/mm2 Conclusion: Our findings point to miR-205-5p as potential biomarker of distant metastases and to miR-145-5p and miR-203-3p as markers of aggressiveness in melanoma.
2019
utaneous melanoma; metastasis; metastatic melanoma; microRNAs; molecular biomarkers; thick melanoma; thin melanoma
01 Pubblicazione su rivista::01a Articolo in rivista
MiRNAs as potential prognostic biomarkers for metastasis in thin and thick primary cutaneous melanomas / Valentini, Virginia; Zelli, Veronica; Gaggiano, Emanuela; Silvestri, Valentina; Rizzolo, Piera; Bucalo, Agostino; Calvieri, Stefano; Grassi, Sara; Frascione, Pasquale; Pietro, Donati; Soda, Giuseppe; Ottini, Laura; Richetta, Antonio Giovanni. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - 39:8(2019), pp. 4085-4093. [10.21873/anticanres.13566]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1305702
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