A pilot study was carried out on five obese/overweight patients suffering from metabolic syndrome, with the aim to evaluate postprandial effects of high fat/high glycemic load meals enriched by blueberries. Postprandial urine samples were analyzed by 1H-NMR spectroscopy after 2 and 4 h from ingestion to identify potential markers of blueberry intake. Significant decrease of methylamines, acetoacetate, acetone and succinate, known indicators of type 2 diabetes mellitus, were observed after the intake of meals enriched with blueberries. On the other hand, an accumulation of p-hydroxyphenyl-acetic acid and 3-(3'-hydroxyphenyl)-3-hydropropionic acid originating from gut microbial dehydrogenation of proanthocyanidins and procyanidins was detected. Real-time PCR-analysis of mRNAs obtained from mononuclear blood cells showed significant changes in cytokine gene expression levels after meals integrated with blueberries. In particular, the mRNAs expression of interleukin-6 (IL-6) and Transforming Growth Factor-β (TGF-β), pro and anti-inflammation cytokines, respectively, significantly decreased and increased after blueberry supplementation, indicating a positive impact of blueberry ingestion in the reduction of risk of inflammation. The combined analysis of the urine metabolome and clinical markers represents a promising approach in monitoring the metabolic impact of blueberries in persons with metabolic syndrome.

Blueberry-Based Meals for Obese Patients with Metabolic Syndrome: A Multidisciplinary Metabolomic Pilot Study / Sobolev, Anatoly Petrovich; Ciampa, Alessandra; Ingallina, Cinzia; Mannina, Luisa; Capitani, Donatella; Ernesti, Ilaria; Maggi, Elisa; Businaro, Rita; Del Ben, Maria; Engel, Petra; Giusti, Anna Maria; Donini, Lorenzo M; Pinto, Alessandro. - In: METABOLITES. - ISSN 2218-1989. - 9:7(2019). [10.3390/metabo9070138]

Blueberry-Based Meals for Obese Patients with Metabolic Syndrome: A Multidisciplinary Metabolomic Pilot Study

CIAMPA, ALESSANDRA
Visualization
;
Ingallina, Cinzia
Validation
;
Mannina, Luisa
Project Administration
;
Capitani, Donatella
Writing – Review & Editing
;
Ernesti, Ilaria;Maggi, Elisa
Methodology
;
Businaro, Rita
Funding Acquisition
;
Del Ben, Maria
Funding Acquisition
;
Giusti, Anna Maria
Data Curation
;
Donini, Lorenzo M
Conceptualization
;
Pinto, Alessandro
Writing – Original Draft Preparation
2019

Abstract

A pilot study was carried out on five obese/overweight patients suffering from metabolic syndrome, with the aim to evaluate postprandial effects of high fat/high glycemic load meals enriched by blueberries. Postprandial urine samples were analyzed by 1H-NMR spectroscopy after 2 and 4 h from ingestion to identify potential markers of blueberry intake. Significant decrease of methylamines, acetoacetate, acetone and succinate, known indicators of type 2 diabetes mellitus, were observed after the intake of meals enriched with blueberries. On the other hand, an accumulation of p-hydroxyphenyl-acetic acid and 3-(3'-hydroxyphenyl)-3-hydropropionic acid originating from gut microbial dehydrogenation of proanthocyanidins and procyanidins was detected. Real-time PCR-analysis of mRNAs obtained from mononuclear blood cells showed significant changes in cytokine gene expression levels after meals integrated with blueberries. In particular, the mRNAs expression of interleukin-6 (IL-6) and Transforming Growth Factor-β (TGF-β), pro and anti-inflammation cytokines, respectively, significantly decreased and increased after blueberry supplementation, indicating a positive impact of blueberry ingestion in the reduction of risk of inflammation. The combined analysis of the urine metabolome and clinical markers represents a promising approach in monitoring the metabolic impact of blueberries in persons with metabolic syndrome.
1H-NMR; blueberries; cytokines; metabolic syndrome; urine
01 Pubblicazione su rivista::01a Articolo in rivista
Blueberry-Based Meals for Obese Patients with Metabolic Syndrome: A Multidisciplinary Metabolomic Pilot Study / Sobolev, Anatoly Petrovich; Ciampa, Alessandra; Ingallina, Cinzia; Mannina, Luisa; Capitani, Donatella; Ernesti, Ilaria; Maggi, Elisa; Businaro, Rita; Del Ben, Maria; Engel, Petra; Giusti, Anna Maria; Donini, Lorenzo M; Pinto, Alessandro. - In: METABOLITES. - ISSN 2218-1989. - 9:7(2019). [10.3390/metabo9070138]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1299482
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