In this work, the authors prepared and characterized two different graphene oxides: one chemically synthesized (GO sample) and the other one electrochemically synthesized (GO (LiCl) ). Both samples were fully characterized with atomic force microscopy (AFM), Raman and Fourier transform infrared (FTIR) spectroscopies, X-ray photo electron spectroscopy (XPS), thermal analysis (TG/DTA), and Z-potential. The antibacterial properties of both graphene oxides were studied using Gram-negative Escherichia coli ATCC 25922 and Gram-positive Staphylococcus aureus ATCC 25923 by spectrophotometer and viable cell count as indirect and direct methods, respectively. Results demonstrated that the GO (LiCl) exhibited a significant antibacterial activity compared to GO that showed a bacteriostatic effect on both pathogens. Electron microscopy analysis confirmed the antibacterial effects of both graphene oxides toward the pathogens, especially working at 80 μg/mL, for 24 h. Additional studies were also performed and both GO samples were not cytotoxic at 2 μg/mL toward neuroblastoma cells. Moreover, 2 μg of GO was suitable to carry the minimum effective dose (5.74 ng/mL) of kinase inhibitor S29 (1-(2-chloro-2-(4-chlorophenyl)ethyl)-N-(4-fluorobenzyl)-1H-pyrazolo[3,4-d] pyrimidin-4-amine), providing negligible side effects related to the S29 treatment (this latter being specifically active on the neuroblastoma cell lines (SK-N-BE(2))).

Functionalized graphene derivatives: antibacterial properties and cytotoxicity / Valentini, F.; Calcaterra, A.; Ruggiero, V.; Pichichero, E.; Martino, A.; Iosi, F.; Bertuccini, L.; Antonaroli, S.; Mardente, S.; Zicari, A.; Mari, E.; Iovenitti, G.; Leone, G.; Botta, M.; Talamo, M.. - In: JOURNAL OF NANOMATERIALS. - ISSN 1687-4110. - 2019:(2019). [10.1155/2019/2752539]

Functionalized graphene derivatives: antibacterial properties and cytotoxicity

Calcaterra A.
Membro del Collaboration Group
;
Mardente S.
Membro del Collaboration Group
;
Zicari A.
Membro del Collaboration Group
;
Mari E.
Membro del Collaboration Group
;
Botta M.
Membro del Collaboration Group
;
Talamo M.
2019

Abstract

In this work, the authors prepared and characterized two different graphene oxides: one chemically synthesized (GO sample) and the other one electrochemically synthesized (GO (LiCl) ). Both samples were fully characterized with atomic force microscopy (AFM), Raman and Fourier transform infrared (FTIR) spectroscopies, X-ray photo electron spectroscopy (XPS), thermal analysis (TG/DTA), and Z-potential. The antibacterial properties of both graphene oxides were studied using Gram-negative Escherichia coli ATCC 25922 and Gram-positive Staphylococcus aureus ATCC 25923 by spectrophotometer and viable cell count as indirect and direct methods, respectively. Results demonstrated that the GO (LiCl) exhibited a significant antibacterial activity compared to GO that showed a bacteriostatic effect on both pathogens. Electron microscopy analysis confirmed the antibacterial effects of both graphene oxides toward the pathogens, especially working at 80 μg/mL, for 24 h. Additional studies were also performed and both GO samples were not cytotoxic at 2 μg/mL toward neuroblastoma cells. Moreover, 2 μg of GO was suitable to carry the minimum effective dose (5.74 ng/mL) of kinase inhibitor S29 (1-(2-chloro-2-(4-chlorophenyl)ethyl)-N-(4-fluorobenzyl)-1H-pyrazolo[3,4-d] pyrimidin-4-amine), providing negligible side effects related to the S29 treatment (this latter being specifically active on the neuroblastoma cell lines (SK-N-BE(2))).
Graphene derivatives; cytotoxicity; TK inhibitor
01 Pubblicazione su rivista::01a Articolo in rivista
Functionalized graphene derivatives: antibacterial properties and cytotoxicity / Valentini, F.; Calcaterra, A.; Ruggiero, V.; Pichichero, E.; Martino, A.; Iosi, F.; Bertuccini, L.; Antonaroli, S.; Mardente, S.; Zicari, A.; Mari, E.; Iovenitti, G.; Leone, G.; Botta, M.; Talamo, M.. - In: JOURNAL OF NANOMATERIALS. - ISSN 1687-4110. - 2019:(2019). [10.1155/2019/2752539]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1283466
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