Aspergillus sclerotiorum as a new producer of hemostatically active proteins

The hemostatic system of human blood is the most important system that provides blood flow, which prevents blood loss. Some processes of coagulation, anticoagulation, and thrombolysis are based on action of proteases. Many species of the genus Aspergillus produces proteases acting on proteins of the hemostatic system. These enzymes are capable of cleaving chromogenic peptide substrates specific for hemostatic proteins and activate a number of plasma hemostatic proenzymes (for example, protein C). It was shown that A. sclerotiorum has a high fibrinolytic activity. This may lead to the fact that micromycete may have other proteolytic activities of proteases of the hemostatic system. It was shown that A. sclerotiorum has a high thrombin-like (127 nanomoles pNA/(ml*min)), plasmin-like (194 nanomoles pNA/(ml*min)) activities and activator activity towards protein C (141 nanomoles pNA/(ml*min)). Activator activity towards protein C is significantly higher than that of A. oryzae (31,4 nanomoles pNA/(ml*min)), A. alliaceus (28,4 nanomoles pNA/(ml*min)) and A. nidulans (4,8 nanomoles pNA/(ml*min)).