From transcription, to transport, storage, and translation, RNA depends on association with different RNA-binding proteins (RBPs). Methods based on next-generation sequencing and protein mass-spectrometry have started to unveil genome-wide interactions of RBPs but many aspects still remain out of sight. How many of the binding sites identified in high-throughput screenings are functional? A number of computational methods have been developed to analyze experimental data and to obtain insights into the specificity of protein–RNA interactions. How can theoretical models be exploited to identify RBPs? In addition to oligomeric complexes, protein and RNA molecules can associate into granular assemblies whose physical properties are still poorly understood. What protein features promote granule formation and what effects do these assemblies have on cell function? Here, we describe the newest in silico, in vitro, and in vivo advances in the field of protein–RNA interactions. We also present the challenges that experimental and computational approaches will have to face in future studies. WIREs RNA 2016, 7:793–810. doi: 10.1002/wrna.1378. For further resources related to this article, please visit the WIREs website.

Advances in the characterization of RNA-binding proteins / Marchese, Domenica; de Groot, Natalia Sanchez; Lorenzo Gotor, Nieves; Livi, Carmen Maria; Tartaglia, Gian G.. - In: WILEY INTERDISCIPLINARY REVIEWS. RNA. - ISSN 1757-7004. - 7:6(2016), pp. 793-810. [10.1002/wrna.1378]

Advances in the characterization of RNA-binding proteins

Marchese, Domenica;Tartaglia, Gian G.
2016

Abstract

From transcription, to transport, storage, and translation, RNA depends on association with different RNA-binding proteins (RBPs). Methods based on next-generation sequencing and protein mass-spectrometry have started to unveil genome-wide interactions of RBPs but many aspects still remain out of sight. How many of the binding sites identified in high-throughput screenings are functional? A number of computational methods have been developed to analyze experimental data and to obtain insights into the specificity of protein–RNA interactions. How can theoretical models be exploited to identify RBPs? In addition to oligomeric complexes, protein and RNA molecules can associate into granular assemblies whose physical properties are still poorly understood. What protein features promote granule formation and what effects do these assemblies have on cell function? Here, we describe the newest in silico, in vitro, and in vivo advances in the field of protein–RNA interactions. We also present the challenges that experimental and computational approaches will have to face in future studies. WIREs RNA 2016, 7:793–810. doi: 10.1002/wrna.1378. For further resources related to this article, please visit the WIREs website.
2016
Animals; Binding Sites; Humans; Protein Binding; RNA; RNA-Binding Proteins; Biochemistry; Molecular Biology
01 Pubblicazione su rivista::01a Articolo in rivista
Advances in the characterization of RNA-binding proteins / Marchese, Domenica; de Groot, Natalia Sanchez; Lorenzo Gotor, Nieves; Livi, Carmen Maria; Tartaglia, Gian G.. - In: WILEY INTERDISCIPLINARY REVIEWS. RNA. - ISSN 1757-7004. - 7:6(2016), pp. 793-810. [10.1002/wrna.1378]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1253381
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