Treatment of leukemia cells with 1,25-dihydroxyvitamin D3 may overcome their differentiation block and lead to the transition from myeloblasts to monocytes. To identify microRNA-mRNA networks relevant for myeloid differentiation, we profiled the expression of mRNAs and microRNAs associated to the low- and high-density ribosomal fractions in leukemic cells and in their differentiated monocytic counterpart. Intersection between mRNAs shifted across the fractions after treatment with putative target genes of modulated microRNAs showed a series of molecular networks relevant for the monocyte cell fate determination, as for example the post-transcriptional regulation of the Polo-like kinase 1 (PLK1) by miR-22–3p and let-7e-5p.
Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition / Fontemaggi, Giulia; Bellissimo, Teresa; Donzelli, Sara; Iosue', Ilaria; Benassi, Barbara; Bellotti, Giorgio; Blandino1, Giovanni; Fazi, Francesco. - (2015).
Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition
Teresa Bellissimo;Ilaria Iosue;Francesco Fazi
2015
Abstract
Treatment of leukemia cells with 1,25-dihydroxyvitamin D3 may overcome their differentiation block and lead to the transition from myeloblasts to monocytes. To identify microRNA-mRNA networks relevant for myeloid differentiation, we profiled the expression of mRNAs and microRNAs associated to the low- and high-density ribosomal fractions in leukemic cells and in their differentiated monocytic counterpart. Intersection between mRNAs shifted across the fractions after treatment with putative target genes of modulated microRNAs showed a series of molecular networks relevant for the monocyte cell fate determination, as for example the post-transcriptional regulation of the Polo-like kinase 1 (PLK1) by miR-22–3p and let-7e-5p.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.