Neuropeptide Y (NPY), a powerful neurotransmitter of the central nervous system, is a key regulator of angiogenesis and biology of adipose depots. Intriguingly, its peripheral vascular and angiogenic powerful activity is strictly associated to platelets, which are source of clinical hemoderivates, such as platelet lysate (PL), routinely employed in several clinical applications as wound healing, and to preserve ex vivo the progenitor properties of the adipose stromal cells pool. So far, the presence of NPY in PL and its biological effects on the adipose stromal cell fraction (ASCs) have never been investigated. Here, we aimed to identify endogenous sources of NPY such as PL-based preparations and to investigate which biological properties PL-derived NPY is able to exert on ASCs. The results show that PL contains a high amount of NPY, which is in part also excreted by ASCs when stimulated with PL. The protein levels of the three main NPY subtype receptors (Y1, Y2, Y5) are unaltered by stimulation of ASCs with PL, but their inhibition through selective pharmacological antagonists, considerably enhances migration, and a parallel reduction of angiogenic features of ASCs including decrease in VEGF mRNA and intracellular calcium levels, both downstream targets of NPY. The expression of VEGF and NPY is enhanced within the sites of neovascularisation of difficult wounds in patients after treatment with leuco-platelet concentrates. Our data highlight the presence of NPY in PL preparations and its peripheral effects on adipose progenitors.

Platelet lysate-derived neuropeptide y influences migration and angiogenesis of human adipose tissue-derived stromal cells / Businaro, Rita; Scaccia, Eleonora; Bordin, Antonella; Pagano, Francesca; Corsi, Mariangela; Siciliano, Camilla; Capoano, Raffaele; Procaccini, Eugenio; Salvati, Bruno; Petrozza, Vincenzo; Pierangelatotta, ; Teresa Vietri, Maria; Frati, Giacomo; DE FALCO, Elena. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - ELETTRONICO. - 8:1(2018), pp. 1-12. [10.1038/s41598-018-32623-8]

Platelet lysate-derived neuropeptide y influences migration and angiogenesis of human adipose tissue-derived stromal cells

Rita Businaro
Co-primo
;
Antonella Bordin;Francesca Pagano;Mariangela Corsi;Camilla Siciliano;Raffaele Capoano;Bruno Salvati;Vincenzo Petrozza;Giacomo Frati;Elena De Falco
Supervision
2018

Abstract

Neuropeptide Y (NPY), a powerful neurotransmitter of the central nervous system, is a key regulator of angiogenesis and biology of adipose depots. Intriguingly, its peripheral vascular and angiogenic powerful activity is strictly associated to platelets, which are source of clinical hemoderivates, such as platelet lysate (PL), routinely employed in several clinical applications as wound healing, and to preserve ex vivo the progenitor properties of the adipose stromal cells pool. So far, the presence of NPY in PL and its biological effects on the adipose stromal cell fraction (ASCs) have never been investigated. Here, we aimed to identify endogenous sources of NPY such as PL-based preparations and to investigate which biological properties PL-derived NPY is able to exert on ASCs. The results show that PL contains a high amount of NPY, which is in part also excreted by ASCs when stimulated with PL. The protein levels of the three main NPY subtype receptors (Y1, Y2, Y5) are unaltered by stimulation of ASCs with PL, but their inhibition through selective pharmacological antagonists, considerably enhances migration, and a parallel reduction of angiogenic features of ASCs including decrease in VEGF mRNA and intracellular calcium levels, both downstream targets of NPY. The expression of VEGF and NPY is enhanced within the sites of neovascularisation of difficult wounds in patients after treatment with leuco-platelet concentrates. Our data highlight the presence of NPY in PL preparations and its peripheral effects on adipose progenitors.
2018
adipose stromal cells; human platelet lysate; neuropeptide Y; vascular endothelial growth factor; angiogenesis; chemotaxis
01 Pubblicazione su rivista::01a Articolo in rivista
Platelet lysate-derived neuropeptide y influences migration and angiogenesis of human adipose tissue-derived stromal cells / Businaro, Rita; Scaccia, Eleonora; Bordin, Antonella; Pagano, Francesca; Corsi, Mariangela; Siciliano, Camilla; Capoano, Raffaele; Procaccini, Eugenio; Salvati, Bruno; Petrozza, Vincenzo; Pierangelatotta, ; Teresa Vietri, Maria; Frati, Giacomo; DE FALCO, Elena. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - ELETTRONICO. - 8:1(2018), pp. 1-12. [10.1038/s41598-018-32623-8]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1157717
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