Different acellular assays are currently used for the determination of the oxidative potential (OP) of particular matter (PM). In order to better understand the existing correlations between OP and the PM generated by specific emission sources, dusts generated from seven different sources (urban dust, soil, road dust, brake dust, pellet ash, coke, and desert dust) were chemically characterised and analysed using three OP assays: dithiothreitol (DTT), acid ascorbic (AA) and 2′,7′-dichlorofluorescin (DCFH). In agreement with some previous literature studies, these assays provided very different results for each selected dust: their reliability as a proxy of ROS generation in biological system need then to be further investigated. When applied to the soluble fraction of these dusts, the DTT and AA assays were particularly sensitive toward pellet ash, while the highest OPDCFH values were measured for road dust. Multivariate analysis was used to confirm the correlations between the OP assay results and the chemical characteristics of the dust soluble fractions. DTT and AA assays appeared mostly influenced by organic and inorganic components, respectively. In the case of the DCFH assay, no clear correlations were highlighted, but, differently from the other assays, relevant OP values were measured also for dust of crustal origin. The insoluble fraction of the dusts, which is often not considered in this kind of study, generated, for all the considered assays, OP values even higher than those related to the soluble fraction.
Oxidative potential of particulate matter collected at industrial and urban sites / Simonetti, Giulia; Conte, Elena; Massimi, Lorenzo; Canepari, Silvia. - STAMPA. - (2018), pp. 1-1. (Intervento presentato al convegno SETAC 2018 - SETAC (Society of Environmental Toxicology and Chemistry Europe) Europe 28th Annual Meeting tenutosi a La Nuvola Rome Convention Centre, Rome (Italy)).
Oxidative potential of particulate matter collected at industrial and urban sites
Giulia Simonetti;Elena Conte;Lorenzo Massimi
;Silvia Canepari
2018
Abstract
Different acellular assays are currently used for the determination of the oxidative potential (OP) of particular matter (PM). In order to better understand the existing correlations between OP and the PM generated by specific emission sources, dusts generated from seven different sources (urban dust, soil, road dust, brake dust, pellet ash, coke, and desert dust) were chemically characterised and analysed using three OP assays: dithiothreitol (DTT), acid ascorbic (AA) and 2′,7′-dichlorofluorescin (DCFH). In agreement with some previous literature studies, these assays provided very different results for each selected dust: their reliability as a proxy of ROS generation in biological system need then to be further investigated. When applied to the soluble fraction of these dusts, the DTT and AA assays were particularly sensitive toward pellet ash, while the highest OPDCFH values were measured for road dust. Multivariate analysis was used to confirm the correlations between the OP assay results and the chemical characteristics of the dust soluble fractions. DTT and AA assays appeared mostly influenced by organic and inorganic components, respectively. In the case of the DCFH assay, no clear correlations were highlighted, but, differently from the other assays, relevant OP values were measured also for dust of crustal origin. The insoluble fraction of the dusts, which is often not considered in this kind of study, generated, for all the considered assays, OP values even higher than those related to the soluble fraction.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
