This paper presents a lab-on-chip system suitable for real-time polymerase chain reaction (RTPCR) to quantify the number of DNA copies in samples, taking advantage of the combination of thin film technologies on the same glass substrate. A thin film metal heater, deposited on one side of a glass substrate, provides the thermal energy for the DNA treatment, while amorphous silicon (a-Si:H) photosensors, deposited on the other glass side, monitor the DNA amplification. Indeed, the glass is optically coupled with another glass hosting the microfluidic network, where the PCR occurs.
Lab-on-Chip system based on thin film technologies for real-time PCR / Caputo, Domenico; Petrucci, Giulia; Costantini, Francesca; Lovecchio, Nicola; Nardecchia, Marco; Nascetti, Augusto; DE CESARE, Giampiero. - ELETTRONICO. - (2017), p. 025. (Intervento presentato al convegno 5th International Conference on Bio-Sensing Technology tenutosi a Riva del Garda, Italy nel 7 - 10 May 2017).
Lab-on-Chip system based on thin film technologies for real-time PCR
CAPUTO, Domenico;PETRUCCI, GIULIA;COSTANTINI, FRANCESCA;LOVECCHIO, NICOLA;NARDECCHIA, MARCO;NASCETTI, Augusto;DE CESARE, Giampiero
2017
Abstract
This paper presents a lab-on-chip system suitable for real-time polymerase chain reaction (RTPCR) to quantify the number of DNA copies in samples, taking advantage of the combination of thin film technologies on the same glass substrate. A thin film metal heater, deposited on one side of a glass substrate, provides the thermal energy for the DNA treatment, while amorphous silicon (a-Si:H) photosensors, deposited on the other glass side, monitor the DNA amplification. Indeed, the glass is optically coupled with another glass hosting the microfluidic network, where the PCR occurs.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
