This paper presents a lab-on-chip system suitable for real-time polymerase chain reaction (RTPCR) to quantify the number of DNA copies in samples, taking advantage of the combination of thin film technologies on the same glass substrate. A thin film metal heater, deposited on one side of a glass substrate, provides the thermal energy for the DNA treatment, while amorphous silicon (a-Si:H) photosensors, deposited on the other glass side, monitor the DNA amplification. Indeed, the glass is optically coupled with another glass hosting the microfluidic network, where the PCR occurs.

Lab-on-Chip system based on thin film technologies for real-time PCR / Caputo, Domenico; Petrucci, Giulia; Costantini, Francesca; Lovecchio, Nicola; Nardecchia, Marco; Nascetti, Augusto; DE CESARE, Giampiero. - ELETTRONICO. - (2017), p. 025. (Intervento presentato al convegno 5th International Conference on Bio-Sensing Technology tenutosi a Riva del Garda, Italy nel 7 - 10 May 2017).

Lab-on-Chip system based on thin film technologies for real-time PCR

CAPUTO, Domenico;PETRUCCI, GIULIA;COSTANTINI, FRANCESCA;LOVECCHIO, NICOLA;NARDECCHIA, MARCO;NASCETTI, Augusto;DE CESARE, Giampiero
2017

Abstract

This paper presents a lab-on-chip system suitable for real-time polymerase chain reaction (RTPCR) to quantify the number of DNA copies in samples, taking advantage of the combination of thin film technologies on the same glass substrate. A thin film metal heater, deposited on one side of a glass substrate, provides the thermal energy for the DNA treatment, while amorphous silicon (a-Si:H) photosensors, deposited on the other glass side, monitor the DNA amplification. Indeed, the glass is optically coupled with another glass hosting the microfluidic network, where the PCR occurs.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/985835
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