The purpose of this study was to analyze the physiological features of peripheral blood mononuclear cells (PBMCs) isolated from healthy female trekkers before and after physical activity carried out under both normoxia (low altitude, < 2000 m a.s.l.) and hypobaric hypoxia (high altitude, > 3700 m a.s.l.). The experimental design was to differentiate effects induced by exercise and those related to external environmental conditions. PBMCs were isolated from seven female subjects before and after each training period. The PBMCs were phenotypically and functionally characterized using fluorimetric and densitometric analyses, to determine cellular activation, and their intracellular Ca2+ levels and oxidative status. After a period of normoxic physical exercise, the PBMCs showed an increase in fully activated T lymphocytes (CD3+CD69+) and a reduction in intracellular Ca2+ levels. On the other hand, with physical exercise performed under hypobaric hypoxia, there was a reduction in T lymphocytes and an increase in nonactivated B lymphocytes, accompanied by a reduction in O2 − levels in the mitochondria. These outcomes reveal that in women, low- to moderate-intensity aerobic trekking induces CD69 T cell activation and promotes anti-stress effects on the high-altitude-induced impairment of the immune responses and the oxidative balance.

Responses of peripheral blood mononuclear cells to moderate exercise and hypoxia / Morabito, C.; Lanuti, P.; Caprara, G. A.; Guarnieri, S.; Verratti, V.; Ricci, G.; Catizone, Angiolina; Marchisio, M.; Fanò Illic, G.; Mariggiò, M. A.. - In: SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS. - ISSN 0905-7188. - 26:10(2016), pp. 1188-1199. [10.1111/sms.12557]

Responses of peripheral blood mononuclear cells to moderate exercise and hypoxia

CATIZONE, Angiolina;
2016

Abstract

The purpose of this study was to analyze the physiological features of peripheral blood mononuclear cells (PBMCs) isolated from healthy female trekkers before and after physical activity carried out under both normoxia (low altitude, < 2000 m a.s.l.) and hypobaric hypoxia (high altitude, > 3700 m a.s.l.). The experimental design was to differentiate effects induced by exercise and those related to external environmental conditions. PBMCs were isolated from seven female subjects before and after each training period. The PBMCs were phenotypically and functionally characterized using fluorimetric and densitometric analyses, to determine cellular activation, and their intracellular Ca2+ levels and oxidative status. After a period of normoxic physical exercise, the PBMCs showed an increase in fully activated T lymphocytes (CD3+CD69+) and a reduction in intracellular Ca2+ levels. On the other hand, with physical exercise performed under hypobaric hypoxia, there was a reduction in T lymphocytes and an increase in nonactivated B lymphocytes, accompanied by a reduction in O2 − levels in the mitochondria. These outcomes reveal that in women, low- to moderate-intensity aerobic trekking induces CD69 T cell activation and promotes anti-stress effects on the high-altitude-induced impairment of the immune responses and the oxidative balance.
2016
exercise training in women; hypobaric hypoxia; intracellular calcium; oxidative stress; adult; altitude; antigens, CD; CD3; differentiation, T-Lymphocyte; B-Lymphocytes; calcium; exercise; female; humans; hypoxia; lectins, C-type; lymphocyte activation; lymphocyte count; mitochondria; mountaineering; oxidative stress; oxygen; physical conditioning; Human; reactive oxygen species; T-Lymphocytes; orthopedics and sports medicine; physical therapy; sports therapy and rehabilitation
01 Pubblicazione su rivista::01a Articolo in rivista
Responses of peripheral blood mononuclear cells to moderate exercise and hypoxia / Morabito, C.; Lanuti, P.; Caprara, G. A.; Guarnieri, S.; Verratti, V.; Ricci, G.; Catizone, Angiolina; Marchisio, M.; Fanò Illic, G.; Mariggiò, M. A.. - In: SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS. - ISSN 0905-7188. - 26:10(2016), pp. 1188-1199. [10.1111/sms.12557]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/980790
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