The last 25 years have seen an ever-growing use of the erythrocyte micronucleus test for measuring damage to mammalian chromosomes in vivo. In addition, staining micronuclei with antikinetochore antibodies from CREST serum discriminates aneugenic from clastogenic damage. The use of the micronucleus test in rats, however, has been problematic because the spleen of adult rats efficiently removes micronucleated erythrocytes from the blood. In the present study, we have treated 5-day-old rats with either X-rays (a clastogen) or vinblastine (an aneugen) and measured micronuclei in erythrocytes from the blood and liver. Each treatment increased the frequency of micronuclei in both tissues, with the percentages of CREST-staining micronuclei reflecting the mechanism of micronucleus induction by the two agents. The results indicate that performing the micronucleus assay in the liver and peripheral blood of 5-day-old rats may be a useful approach for detecting the in vivo genotoxicity of chemical and physical agents.

Detection of clastogenic and aneugenic damage in newborn rats / Ion, Udroiu; Luisa Anna, Ieradi; Cristaldi, Mauro; Caterina, Tanzarella. - In: ENVIRONMENTAL AND MOLECULAR MUTAGENESIS. - ISSN 0893-6692. - STAMPA. - 47:5(2006), pp. 320-324. [10.1002/em.20209]

Detection of clastogenic and aneugenic damage in newborn rats

CRISTALDI, Mauro;
2006

Abstract

The last 25 years have seen an ever-growing use of the erythrocyte micronucleus test for measuring damage to mammalian chromosomes in vivo. In addition, staining micronuclei with antikinetochore antibodies from CREST serum discriminates aneugenic from clastogenic damage. The use of the micronucleus test in rats, however, has been problematic because the spleen of adult rats efficiently removes micronucleated erythrocytes from the blood. In the present study, we have treated 5-day-old rats with either X-rays (a clastogen) or vinblastine (an aneugen) and measured micronuclei in erythrocytes from the blood and liver. Each treatment increased the frequency of micronuclei in both tissues, with the percentages of CREST-staining micronuclei reflecting the mechanism of micronucleus induction by the two agents. The results indicate that performing the micronucleus assay in the liver and peripheral blood of 5-day-old rats may be a useful approach for detecting the in vivo genotoxicity of chemical and physical agents.
2006
crest; liver; micronuclei; newborn rats; newborn rats.; peripheral blood
01 Pubblicazione su rivista::01a Articolo in rivista
Detection of clastogenic and aneugenic damage in newborn rats / Ion, Udroiu; Luisa Anna, Ieradi; Cristaldi, Mauro; Caterina, Tanzarella. - In: ENVIRONMENTAL AND MOLECULAR MUTAGENESIS. - ISSN 0893-6692. - STAMPA. - 47:5(2006), pp. 320-324. [10.1002/em.20209]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/97990
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 22
  • ???jsp.display-item.citation.isi??? 21
social impact