Abstract The present study demonstrates, in both hybrid NG108-15 and mouse neuroblastoma N18TG2 cells, the presence and regulation of PDE5 mRNA during cell differentiation. PDE5 cDNA probes in Northern blot analysis recognize a V9 kb transcript in bovine lung as well as in mouse neuroblastoma cells. Hybridization on total RNA extracted from dibutyryl- cAMP-treated NG108-15 cells shows a 5-fold increase of PDE5 9 kb mRNA: such an increase is not observed in N18TG2 although we observed a similar increase in the enzymatic activity of both cell lines. Our data demonstrate that PDE5 gene expression can be regulated by cAMP and suggest the existence of a complex regulatory system for PDE5 activity.

cAMP-dependent induction of PDE5 expression in murine neuroblastoma cell differentiation / Giordano, D.; Giorgi, Mauro; Sette, C.; Biagioni, Stefano; Augusti Tocco, G.. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 446:2-3(1999), pp. 218-222. [10.1016/S0014-5793(99)00227-6]

cAMP-dependent induction of PDE5 expression in murine neuroblastoma cell differentiation

GIORGI, MAURO;BIAGIONI, Stefano;
1999

Abstract

Abstract The present study demonstrates, in both hybrid NG108-15 and mouse neuroblastoma N18TG2 cells, the presence and regulation of PDE5 mRNA during cell differentiation. PDE5 cDNA probes in Northern blot analysis recognize a V9 kb transcript in bovine lung as well as in mouse neuroblastoma cells. Hybridization on total RNA extracted from dibutyryl- cAMP-treated NG108-15 cells shows a 5-fold increase of PDE5 9 kb mRNA: such an increase is not observed in N18TG2 although we observed a similar increase in the enzymatic activity of both cell lines. Our data demonstrate that PDE5 gene expression can be regulated by cAMP and suggest the existence of a complex regulatory system for PDE5 activity.
cAMP-dependent expression; Differentiation; Neuroblastoma; NG108-15; PDE5; Biochemistry; Biophysics; Molecular Biology
01 Pubblicazione su rivista::01a Articolo in rivista
cAMP-dependent induction of PDE5 expression in murine neuroblastoma cell differentiation / Giordano, D.; Giorgi, Mauro; Sette, C.; Biagioni, Stefano; Augusti Tocco, G.. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 446:2-3(1999), pp. 218-222. [10.1016/S0014-5793(99)00227-6]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/979893
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