The Doñana National Park (Spain), one of the most important wildlife sites in the West of Europe, was affected (25 April 1998) by the spill of acidic waste rich in toxic metals (mainly zinc, lead, copper, etc.), arsenic and aromatic amines from the Aznalcollar mine accident. Micronuclei test with May Grunwald-Giemsa and with CREST-antikinetocore staining using DAPI as counter-staining were performed on peripheral blood erythrocytes from Algerian mice to evaluate genotoxic damage. Animals were collected in four locations each differently affected by the disaster. Higher frequencies of micronuclei and CREST-positive micronuclei were observed in the sites, which were reached by toxic sludge and contaminated water in comparison with those located within the park. The results obtained applying the two methods indicate that DAPI staining is more sensitive in detecting micronuclei. Genotoxic biomonitoring should be further carried out in the area to control the mutagenetic level in natural populations.

Genotoxic damage in free-living Algerian mouse (Mus spretus) after the Coto Doñana ecological disaster / C., Tanzarella; F., Degrassi; Cristaldi, Mauro; S., Moreno; A., Lascialfari; G., Chiuchiarelli; L. A., Ieradi. - In: ENVIRONMENTAL POLLUTION. - ISSN 0269-7491. - STAMPA. - 115:1(2001), pp. 43-48. [10.1016/S0269-7491(01)00092-6 |]

Genotoxic damage in free-living Algerian mouse (Mus spretus) after the Coto Doñana ecological disaster.

CRISTALDI, Mauro;
2001

Abstract

The Doñana National Park (Spain), one of the most important wildlife sites in the West of Europe, was affected (25 April 1998) by the spill of acidic waste rich in toxic metals (mainly zinc, lead, copper, etc.), arsenic and aromatic amines from the Aznalcollar mine accident. Micronuclei test with May Grunwald-Giemsa and with CREST-antikinetocore staining using DAPI as counter-staining were performed on peripheral blood erythrocytes from Algerian mice to evaluate genotoxic damage. Animals were collected in four locations each differently affected by the disaster. Higher frequencies of micronuclei and CREST-positive micronuclei were observed in the sites, which were reached by toxic sludge and contaminated water in comparison with those located within the park. The results obtained applying the two methods indicate that DAPI staining is more sensitive in detecting micronuclei. Genotoxic biomonitoring should be further carried out in the area to control the mutagenetic level in natural populations.
2001
Bioindicators; CREST-staining; Doñana Park; Micronuclei; Mutagenicity
01 Pubblicazione su rivista::01a Articolo in rivista
Genotoxic damage in free-living Algerian mouse (Mus spretus) after the Coto Doñana ecological disaster / C., Tanzarella; F., Degrassi; Cristaldi, Mauro; S., Moreno; A., Lascialfari; G., Chiuchiarelli; L. A., Ieradi. - In: ENVIRONMENTAL POLLUTION. - ISSN 0269-7491. - STAMPA. - 115:1(2001), pp. 43-48. [10.1016/S0269-7491(01)00092-6 |]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/97717
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