Although a great knowledge about the patho-physiological roles of cholesterol metabolism perturbation in several organs has been reached, scarce information is available on the regulation of cholesterol homeostasis in the brain where this lipid is involved in the maintenance of several of neuronal processes. Currently, no study is available in literature dealing how and if sex and age may modulate the major proteins involved in the regulatory network of cholesterol levels in different brain regions. Here, we investigated the behavior of 3-hydroxy 3-methylglutaryl coenzyme A reductase (HMGR) and low-density lipoprotein receptor (LDLr) in adult (3-month-old) and aged (12-month-old) male and female rats. The analyses were performed in four different brain regions: cortex, brain stem, hippocampus, and cerebellum which represent brain areas characterized by different neuronal cell types, metabolism, cytoarchitecture and white matter composition. The results show that in hippocampus HMGR is lower (30%) in adult female rats than in age-matched males. Differences in LDLr expression are also observable in old females with respect to age-matched males: the protein levels increase (40%) in hippocampus and decrease (20%) in cortex, displaying different mechanisms of regulation. The mechanism underlying the observed modifications are ascribable to Insig-1 and SREBP-1 modulation. The obtained data demonstrate that age- and sex-related differences in cholesterol homeostasis maintenance exist among brain regions, such as the hippocampus and the prefrontal cortex, important for learning, memory and affection. Some of these differences could be at the root of marked gender disparities observed in clinical disease incidence, manifestation, and prognosis.

Analysis of the protein network of cholesterol homeostasis in different brain regions: An age and sex dependent perspective / Segatto, Marco; Di Giovanni, Annalaura; Marino, Maria; Pallottini, Valentina. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - ELETTRONICO. - 228:7(2013), pp. 1561-1567. [10.1002/jcp.24315]

Analysis of the protein network of cholesterol homeostasis in different brain regions: An age and sex dependent perspective

SEGATTO, MARCO;
2013

Abstract

Although a great knowledge about the patho-physiological roles of cholesterol metabolism perturbation in several organs has been reached, scarce information is available on the regulation of cholesterol homeostasis in the brain where this lipid is involved in the maintenance of several of neuronal processes. Currently, no study is available in literature dealing how and if sex and age may modulate the major proteins involved in the regulatory network of cholesterol levels in different brain regions. Here, we investigated the behavior of 3-hydroxy 3-methylglutaryl coenzyme A reductase (HMGR) and low-density lipoprotein receptor (LDLr) in adult (3-month-old) and aged (12-month-old) male and female rats. The analyses were performed in four different brain regions: cortex, brain stem, hippocampus, and cerebellum which represent brain areas characterized by different neuronal cell types, metabolism, cytoarchitecture and white matter composition. The results show that in hippocampus HMGR is lower (30%) in adult female rats than in age-matched males. Differences in LDLr expression are also observable in old females with respect to age-matched males: the protein levels increase (40%) in hippocampus and decrease (20%) in cortex, displaying different mechanisms of regulation. The mechanism underlying the observed modifications are ascribable to Insig-1 and SREBP-1 modulation. The obtained data demonstrate that age- and sex-related differences in cholesterol homeostasis maintenance exist among brain regions, such as the hippocampus and the prefrontal cortex, important for learning, memory and affection. Some of these differences could be at the root of marked gender disparities observed in clinical disease incidence, manifestation, and prognosis.
2013
Aging; Animals; Brain; Brain Stem; Cerebellum; Cerebral Cortex; Cholesterol; Female; Hippocampus; Homeostasis; Hydroxymethylglutaryl CoA Reductases; Intracellular Signaling Peptides and Proteins; Male; Membrane Proteins; Nerve Tissue Proteins; Rats; Rats, Wistar; Receptors, LDL; Sex Characteristics; Sterol Regulatory Element Binding Protein 1; Tissue Distribution; Clinical Biochemistry; Cell Biology; Physiology
01 Pubblicazione su rivista::01a Articolo in rivista
Analysis of the protein network of cholesterol homeostasis in different brain regions: An age and sex dependent perspective / Segatto, Marco; Di Giovanni, Annalaura; Marino, Maria; Pallottini, Valentina. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - ELETTRONICO. - 228:7(2013), pp. 1561-1567. [10.1002/jcp.24315]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/974076
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