For its DNA repair, transcription factor regulation and anti-apoptotic activity, the apurinic/apirimidinic ApeI/Ref-I endonuclease is thought to play a relevant role in human tumorigenesis. In human thyroid tumors, we demonstrated an altered nuclear/cytoplasmic ratio in all the carcinomas examined but not in follicular adenomas. In this study, Ref-I expression and cellular localization were analyzed in a series of human thyroid carcinoma cell lines. We found a reduced nuclear/cytoplasmic ratio in BCPAP, TPCI and ARO cells and not in WRO cells. Such a pattern of expression corresponds to that observed in thyroid tumoral tissues except for the WRO cells which behave as the follicular adenomas rather than carcinomas. Thus, these cell lines represent an excellent in vitro model to analyze the molecular mechanisms involved in Ref-I regulation and activity and clarify its role in thyroid tumorigenesis. ©2001, Editrice Kurtis.

ApeI/Ref-I expression and cellular localization in human thyroid carcinoma cell lines / D., Russo; F., Arturi; S., Bulotta; L., Pellizzari; Filetti, Sebastiano; G., Manzini; G., Damante; G., Tell. - In: JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION. - ISSN 0391-4097. - 24:3(2001), pp. RC10-RC12.

ApeI/Ref-I expression and cellular localization in human thyroid carcinoma cell lines

FILETTI, SEBASTIANO;
2001

Abstract

For its DNA repair, transcription factor regulation and anti-apoptotic activity, the apurinic/apirimidinic ApeI/Ref-I endonuclease is thought to play a relevant role in human tumorigenesis. In human thyroid tumors, we demonstrated an altered nuclear/cytoplasmic ratio in all the carcinomas examined but not in follicular adenomas. In this study, Ref-I expression and cellular localization were analyzed in a series of human thyroid carcinoma cell lines. We found a reduced nuclear/cytoplasmic ratio in BCPAP, TPCI and ARO cells and not in WRO cells. Such a pattern of expression corresponds to that observed in thyroid tumoral tissues except for the WRO cells which behave as the follicular adenomas rather than carcinomas. Thus, these cell lines represent an excellent in vitro model to analyze the molecular mechanisms involved in Ref-I regulation and activity and clarify its role in thyroid tumorigenesis. ©2001, Editrice Kurtis.
apei/ref-i; nuclear/cytoplasmic ratio; redox potential; thyroid tumoral cells
01 Pubblicazione su rivista::01a Articolo in rivista
ApeI/Ref-I expression and cellular localization in human thyroid carcinoma cell lines / D., Russo; F., Arturi; S., Bulotta; L., Pellizzari; Filetti, Sebastiano; G., Manzini; G., Damante; G., Tell. - In: JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION. - ISSN 0391-4097. - 24:3(2001), pp. RC10-RC12.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/96701
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