The peripheral nervous system has an intrinsic regeneration capability. Nevertheless, the nerve regeneration is often not satisfactory, leaving patients with physical morbidity. Schwann Cells (SCs) play a central role in the response of the axon injury, in the removal of myelin debris, in the modulation of inflammatory response, and in the production of growth factors that enhance nerve regeneration. However, SCs show some drawbacks for tissue engineering, as the difficulty in collection and culture and the slow in vitro expansion potential. For this reason, the attention of the researchers has moved toward other cell types presenting best properties for regenerative medicine. The adipose tissue contains mesenchymal stem cells (ASC). Recently, it has demonstrated that ASC can be differentiated in vitro in Schwann-like (dASC). dASC expresses SCs markers and promote neurite outgrowth in vitro. dASCs, as SCs, express receptors for different neurotrasmitters (i.e. GABA, acetylcholine) that can modulate physiological processes such as proliferation, migration and myelination. Acetylcholine (ACh) has the property to control Schwann cell proliferation and differentiation via M2 muscarinic receptors. In present work we characterized the effects mediated by M2 receptors in rat dASC. dASC express all muscarinic receptor subtypes and in particular, M2 subtype. In dASC, M2 agonist APE caused a decreased cell proliferation, the inhibition of cell migration without affecting cell survival. After M2 agonist treatment, we have also observed modulated expression of neurotrophic factors and myelin proteins. Considering the ability of dASC to respond to cholinergic stimuli, we are setting up co-cultures of sensory neurons/dASCs in order to study the in vitro myelination in presence of cholinergic agonists. The data obtained may contribute to identify new therapeutic strategies for peripheral nerve regeneration based on the combination of cell therapy and pharmacological treatments..
The peripheral nervous system has an intrinsic regeneration capability. Nevertheless, the nerve regeneration is often not satisfactory, leaving patients with physical morbidity. Schwann Cells (SCs) play a central role in the response of the axon injury, in the removal of myelin debris, in the modulation of inflammatory response, and in the production of growth factors that enhance nerve regeneration. However, SCs show some drawbacks for tissue engineering, as the difficulty in collection and culture and the slow in vitro expansion potential. For this reason, the attention of the researchers has moved toward other cell types presenting best properties for regenerative medicine. The adipose tissue contains mesenchymal stem cells (ASC). Recently, it has demonstrated that ASC can be differentiated in vitro in Schwann-like (dASC). dASC expresses SCs markers and promote neurite outgrowth in vitro. dASCs, as SCs, express receptors for different neurotrasmitters (i.e. GABA, acetylcholine) that can modulate physiological processes such as proliferation, migration and myelination. Acetylcholine (ACh) has the property to control Schwann cell proliferation and differentiation via M2 muscarinic receptors. In present work we characterized the effects mediated by M2 receptors in rat dASC. dASC express all muscarinic receptor subtypes and in particular, M2 subtype. In dASC, M2 agonist APE caused a decreased cell proliferation, the inhibition of cell migration without affecting cell survival. After M2 agonist treatment, we have also observed modulated expression of neurotrophic factors and myelin proteins. Considering the ability of dASC to respond to cholinergic stimuli, we are setting up co-cultures of sensory neurons/dASCs in order to study the in vitro myelination in presence of cholinergic agonists. The data obtained may contribute to identify new therapeutic strategies for peripheral nerve regeneration based on the combination of cell therapy and pharmacological treatments.
Anti-proliferative and anti-migratory effects mediated by M2 muscarinic receptors in Schwann-like cells induced from adipose mesenchymal stem cells: implication in nerve regeneration / Piovesana, R.; Faroni, A.; Magnaghi, V.; Reid, A.; Tata, Ada Maria. - In: EUROPEAN JOURNAL OF HISTOCHEMISTRY. - ISSN 1121-760X. - STAMPA. - 60/supplement 1:(2016), pp. 11-12. (Intervento presentato al convegno GEI tenutosi a Napoli nel 20-23 Gugno 2016).
Anti-proliferative and anti-migratory effects mediated by M2 muscarinic receptors in Schwann-like cells induced from adipose mesenchymal stem cells: implication in nerve regeneration.
Piovesana R.;TATA, Ada Maria
2016
Abstract
The peripheral nervous system has an intrinsic regeneration capability. Nevertheless, the nerve regeneration is often not satisfactory, leaving patients with physical morbidity. Schwann Cells (SCs) play a central role in the response of the axon injury, in the removal of myelin debris, in the modulation of inflammatory response, and in the production of growth factors that enhance nerve regeneration. However, SCs show some drawbacks for tissue engineering, as the difficulty in collection and culture and the slow in vitro expansion potential. For this reason, the attention of the researchers has moved toward other cell types presenting best properties for regenerative medicine. The adipose tissue contains mesenchymal stem cells (ASC). Recently, it has demonstrated that ASC can be differentiated in vitro in Schwann-like (dASC). dASC expresses SCs markers and promote neurite outgrowth in vitro. dASCs, as SCs, express receptors for different neurotrasmitters (i.e. GABA, acetylcholine) that can modulate physiological processes such as proliferation, migration and myelination. Acetylcholine (ACh) has the property to control Schwann cell proliferation and differentiation via M2 muscarinic receptors. In present work we characterized the effects mediated by M2 receptors in rat dASC. dASC express all muscarinic receptor subtypes and in particular, M2 subtype. In dASC, M2 agonist APE caused a decreased cell proliferation, the inhibition of cell migration without affecting cell survival. After M2 agonist treatment, we have also observed modulated expression of neurotrophic factors and myelin proteins. Considering the ability of dASC to respond to cholinergic stimuli, we are setting up co-cultures of sensory neurons/dASCs in order to study the in vitro myelination in presence of cholinergic agonists. The data obtained may contribute to identify new therapeutic strategies for peripheral nerve regeneration based on the combination of cell therapy and pharmacological treatments..I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
