Abstract Mitochondrial (mt) diseases are devastating neurodegenerative pathologies due to mutations in nuclear or mt genes. Among mtDNA pathogenic mutations, more than one half has been identified in transfer RNA (tRNA) genes. These are responsible for a wide range of pathologies including myopathies, encephalopathies, cardiomyopathies and deafness for which no effective treatment is available at present. Therefore, new strategies to suppress their damaging effects are required to envisage therapeutic approaches for these diseases. Here we report data for carbon nanotubes (CNTs) derivatives showing that the conjugates bearing a specific peptide sequence are able to target the mitochondria in yeast and human monocyte cells while the control derivative without the peptide diffuses into cytoplasm. Moreover the compounds do not affect cellular viability and cytotoxicity both in vitro and in vivo. Toxicity of the constructs is also assessed on the simple pluricellular model Caenorhabditis elegans.

Evaluation of the efficacy of carbon nanotubes for delivering peptides into mitochondria / Ficociello, Graziella; Salemme, Adele; Uccelletti, Daniela; Fiorito, Silvana; Togna, Anna Rita; Vallan, L; Gonzales Dominguez, Jm; Da Ros, T; Francisci, Silvia; Montanari, A.. - In: RSC ADVANCES. - ISSN 2046-2069. - STAMPA. - 6:(2016), pp. 67232-67241. [10.1039/c6ra14254k]

Evaluation of the efficacy of carbon nanotubes for delivering peptides into mitochondria

FICOCIELLO, GRAZIELLA;SALEMME, ADELE;UCCELLETTI, Daniela;FIORITO, Silvana;TOGNA, Anna Rita;FRANCISCI, Silvia;Montanari, A.
2016

Abstract

Abstract Mitochondrial (mt) diseases are devastating neurodegenerative pathologies due to mutations in nuclear or mt genes. Among mtDNA pathogenic mutations, more than one half has been identified in transfer RNA (tRNA) genes. These are responsible for a wide range of pathologies including myopathies, encephalopathies, cardiomyopathies and deafness for which no effective treatment is available at present. Therefore, new strategies to suppress their damaging effects are required to envisage therapeutic approaches for these diseases. Here we report data for carbon nanotubes (CNTs) derivatives showing that the conjugates bearing a specific peptide sequence are able to target the mitochondria in yeast and human monocyte cells while the control derivative without the peptide diffuses into cytoplasm. Moreover the compounds do not affect cellular viability and cytotoxicity both in vitro and in vivo. Toxicity of the constructs is also assessed on the simple pluricellular model Caenorhabditis elegans.
2016
mitochondrial diseases; tRNA mutations; suppressor genes; intracellular delivery; carbon nanotubes; nanomedicine
01 Pubblicazione su rivista::01a Articolo in rivista
Evaluation of the efficacy of carbon nanotubes for delivering peptides into mitochondria / Ficociello, Graziella; Salemme, Adele; Uccelletti, Daniela; Fiorito, Silvana; Togna, Anna Rita; Vallan, L; Gonzales Dominguez, Jm; Da Ros, T; Francisci, Silvia; Montanari, A.. - In: RSC ADVANCES. - ISSN 2046-2069. - STAMPA. - 6:(2016), pp. 67232-67241. [10.1039/c6ra14254k]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/956573
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