Detecting stiff nanoparticles buried in soft biological matrices by atomic force microscopy (AFM) based techniques represents a new frontier in the field of scanning probe microscopies, originally developed as surface characterization methods. Here we report the detection of stiff (magnetic) nanoparticles (NPs) internalized in cells by using contact resonance AFM (CR-AFM) employed as a potentially non-destructive subsurface characterization tool. Magnetite (Fe3O4) NPs were internalized in microglial cells from cerebral cortices of mouse embryos of 18 days by phagocytosis. Nanomechanical imaging of cells was performed by detecting the contact resonance frequencies (CRFs) of an AFM cantilever held in contact with the sample. Agglomerates of NPs internalized in cells were visualized on the basis of the local increase in the contact stiffness with respect to the surrounding biological matrix. A second AFM-based technique for nanomechanical imaging, i.e., HarmoniX™, as well as magnetic force microscopy and light microscopy were used to confirm the CR-AFM results. Thus, CR-AFM was emonstrated as a promising technique for subsurface imaging of nanomaterials in biological samples.

Detection of stiff nanoparticles within cellular structures by contact resonance atomic force microscopy subsurface nanomechanical imaging / Reggente, Melania; Passeri, Daniele; Angeloni, Livia; Scaramuzzo, FRANCESCA ANNA; Barteri, Mario; Angelis, Francesca De; Persiconi, Irene; DE STEFANO, Maria Egle; Rossi, Marco. - In: NANOSCALE. - ISSN 2040-3364. - STAMPA. - 9:(2017), pp. 5671-5676. [10.1039/c7nr01111c]

Detection of stiff nanoparticles within cellular structures by contact resonance atomic force microscopy subsurface nanomechanical imaging

REGGENTE, MELANIA;PASSERI, Daniele;ANGELONI, LIVIA;SCARAMUZZO, FRANCESCA ANNA;BARTERI, Mario;PERSICONI, IRENE;DE STEFANO, Maria Egle;ROSSI, Marco
2017

Abstract

Detecting stiff nanoparticles buried in soft biological matrices by atomic force microscopy (AFM) based techniques represents a new frontier in the field of scanning probe microscopies, originally developed as surface characterization methods. Here we report the detection of stiff (magnetic) nanoparticles (NPs) internalized in cells by using contact resonance AFM (CR-AFM) employed as a potentially non-destructive subsurface characterization tool. Magnetite (Fe3O4) NPs were internalized in microglial cells from cerebral cortices of mouse embryos of 18 days by phagocytosis. Nanomechanical imaging of cells was performed by detecting the contact resonance frequencies (CRFs) of an AFM cantilever held in contact with the sample. Agglomerates of NPs internalized in cells were visualized on the basis of the local increase in the contact stiffness with respect to the surrounding biological matrix. A second AFM-based technique for nanomechanical imaging, i.e., HarmoniX™, as well as magnetic force microscopy and light microscopy were used to confirm the CR-AFM results. Thus, CR-AFM was emonstrated as a promising technique for subsurface imaging of nanomaterials in biological samples.
Nanoparticles; atomic force microscopy; Microglia; nanomechanical imaging
01 Pubblicazione su rivista::01a Articolo in rivista
Detection of stiff nanoparticles within cellular structures by contact resonance atomic force microscopy subsurface nanomechanical imaging / Reggente, Melania; Passeri, Daniele; Angeloni, Livia; Scaramuzzo, FRANCESCA ANNA; Barteri, Mario; Angelis, Francesca De; Persiconi, Irene; DE STEFANO, Maria Egle; Rossi, Marco. - In: NANOSCALE. - ISSN 2040-3364. - STAMPA. - 9:(2017), pp. 5671-5676. [10.1039/c7nr01111c]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/956443
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