Heparin-induced thrombocytopenia (HIT) is a thrombotic complication of heparin therapy. The most used functional method for HIT diagnosis is serotonin release (SRA). A different functional method based on ATP release with luciferine/luciferase long life and stable luminescent signal is used here, which is shown to be comparable for accuracy with SRA in both negative (patients 4Ts ≤3, and negative for both anti-PF4-heparin immunoassay and SRA) and positive (4Ts >3, and positive for both PF4/heparin antibodies and SRA) patients. Our results show that ATP release is higher in washed platelets activated by sera from positive patients than in platelets activated by sera from negative patients. In conclusion, we demonstrate that ATP release assay is a valid alternative method to SRA for the identification of pathogenic antiPF4/heparin antibodies.

New platelet functional method for identification of pathogenic antibodies in HIT patients / Guarino, MARIA LUISA; Massimi, Isabella; Mardente, Stefania; Lappa, A.; Donfrancesco, S.; Visentin, G. P.; Pulcinelli, FABIO MARIA. - In: PLATELETS. - ISSN 0953-7104. - STAMPA. - (2017), pp. 728-730. [10.1080/09537104.2017.1293803]

New platelet functional method for identification of pathogenic antibodies in HIT patients

GUARINO, MARIA LUISA;MASSIMI, ISABELLA;MARDENTE, Stefania;PULCINELLI, FABIO MARIA
2017

Abstract

Heparin-induced thrombocytopenia (HIT) is a thrombotic complication of heparin therapy. The most used functional method for HIT diagnosis is serotonin release (SRA). A different functional method based on ATP release with luciferine/luciferase long life and stable luminescent signal is used here, which is shown to be comparable for accuracy with SRA in both negative (patients 4Ts ≤3, and negative for both anti-PF4-heparin immunoassay and SRA) and positive (4Ts >3, and positive for both PF4/heparin antibodies and SRA) patients. Our results show that ATP release is higher in washed platelets activated by sera from positive patients than in platelets activated by sera from negative patients. In conclusion, we demonstrate that ATP release assay is a valid alternative method to SRA for the identification of pathogenic antiPF4/heparin antibodies.
2017
ATP release; HIT; Hematology
01 Pubblicazione su rivista::01a Articolo in rivista
New platelet functional method for identification of pathogenic antibodies in HIT patients / Guarino, MARIA LUISA; Massimi, Isabella; Mardente, Stefania; Lappa, A.; Donfrancesco, S.; Visentin, G. P.; Pulcinelli, FABIO MARIA. - In: PLATELETS. - ISSN 0953-7104. - STAMPA. - (2017), pp. 728-730. [10.1080/09537104.2017.1293803]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/956293
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