Antimicrobial peptides (AMPs) are produced by all living organisms as a first line defense against invading microorganisms. In Amphibia, they are mainly present in the skin to form a protective chemical barrier between tissues and external environment. Esculentin-1a(1-21)NH2 [Esc(1-21)] is an amphibian skin-derived AMP with a broad spectrum of activity . Unlike the human skin AMP LL-37, Esc(1-21) preserves its antimicrobial activity in biological fluids and is significantly less toxic at high concentrations. By using an in vitro modified scratch assay employing special cell-culture inserts, we found that Esc(1-21) significantly stimulates migration, but not proliferation, of immortalized human keratinocytes (HaCaT cells), more efficiently than LL-37. Importantly, this activity is preserved also on primary human epidermal keratinocytes . Moreover, as previously shown for LL-37, we demonstrated that the Esc(1-21)-induced cell migration involves the activation of the epidermal growth factor receptor and STAT3 protein. Typical morphological changes associated with a migratory phenotype were detected in HaCaT cells upon treatment with Esc(1-21) . These results focus on the wound healing-modulatory properties of Esc(1-21) and suggest it as a novel candidate promoter of skin re-epithelialisation, especially in the management of chronic human skin ulcers.
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|Titolo:||The Antimicrobial Peptide Esculentin-1a(1-21)NH2: A Novel Promoter of Human Skin Wound Healing?|
|Data di pubblicazione:||2016|
|Appartiene alla tipologia:||04a Atto di comunicazione a congresso|