Human umbilical cord blood (CB) has attracted much attention as a reservoir for functional hematopoietic stem and progenitor cells, and, recently, as a source of blood-borne fibroblasts (CB-BFs). Previously, we demonstrated that bone marrow stromal cell (BMSC) and CB-BF pellet cultures make cartilage in vitro. Furthermore, upon in vivo transplantation, BMSC pellets remodelled into miniature bone/marrow organoids. Using this in vivo model, we asked whether CB-BF populations that express characteristics of the hematopoietic stem cell (HSC) niche contain precursors that reform the niche. CB ossicles were regularly observed upon transplantation. Compared with BM ossicles, CB ossicles showed a predominance of red marrow over yellow marrow, as demonstrated by histomorphological analyses and the number of hematopoietic cells isolated within ossicles. Marrow cavities from CB and BM ossicles included donor-derived CD146-expressing osteoprogenitors and host-derived mature hematopoietic cells, clonogenic lineage-committed progenitors and HSCs. Furthermore, human CD34+ cells transplanted into ossicle-bearing mice engrafted and maintained human HSCs in the niche. Our data indicate that CB- BFs are able to recapitulate the conditions by which the bone marrow microenvironment is formed and establish complete HSC niches, which are functionally supportive of hematopoietic tissue.

Human umbilical cord blood-borne fibroblasts contain marrow niche precursors that form a bone/marrow organoid in vivo / Pievani, Alice; Sacchetti, Benedetto; Corsi, Alessandro; Rambaldi, Benedetta; Donsante, Samantha; Scagliotti, Valeria; Vergani, Patrizia; Remoli, Cristina; Robey, Pamela G.; Riminucci, Mara; Serafini, Marta; Biondi, Andrea. - In: DEVELOPMENT. - ISSN 0950-1991. - STAMPA. - 144:6(2017), pp. 1035-1044. [10.1242/dev.142836]

Human umbilical cord blood-borne fibroblasts contain marrow niche precursors that form a bone/marrow organoid in vivo

SACCHETTI, Benedetto;CORSI, ALESSANDRO;DONSANTE, SAMANTHA;REMOLI, CRISTINA;RIMINUCCI, MARA;BIONDI, Andrea
2017

Abstract

Human umbilical cord blood (CB) has attracted much attention as a reservoir for functional hematopoietic stem and progenitor cells, and, recently, as a source of blood-borne fibroblasts (CB-BFs). Previously, we demonstrated that bone marrow stromal cell (BMSC) and CB-BF pellet cultures make cartilage in vitro. Furthermore, upon in vivo transplantation, BMSC pellets remodelled into miniature bone/marrow organoids. Using this in vivo model, we asked whether CB-BF populations that express characteristics of the hematopoietic stem cell (HSC) niche contain precursors that reform the niche. CB ossicles were regularly observed upon transplantation. Compared with BM ossicles, CB ossicles showed a predominance of red marrow over yellow marrow, as demonstrated by histomorphological analyses and the number of hematopoietic cells isolated within ossicles. Marrow cavities from CB and BM ossicles included donor-derived CD146-expressing osteoprogenitors and host-derived mature hematopoietic cells, clonogenic lineage-committed progenitors and HSCs. Furthermore, human CD34+ cells transplanted into ossicle-bearing mice engrafted and maintained human HSCs in the niche. Our data indicate that CB- BFs are able to recapitulate the conditions by which the bone marrow microenvironment is formed and establish complete HSC niches, which are functionally supportive of hematopoietic tissue.
2017
Bone marrow organoid; Cord blood-borne fibroblasts; Hematopoietic stem cell niche; Umbilical cord blood; Molecular Biology; Developmental Biology
01 Pubblicazione su rivista::01a Articolo in rivista
Human umbilical cord blood-borne fibroblasts contain marrow niche precursors that form a bone/marrow organoid in vivo / Pievani, Alice; Sacchetti, Benedetto; Corsi, Alessandro; Rambaldi, Benedetta; Donsante, Samantha; Scagliotti, Valeria; Vergani, Patrizia; Remoli, Cristina; Robey, Pamela G.; Riminucci, Mara; Serafini, Marta; Biondi, Andrea. - In: DEVELOPMENT. - ISSN 0950-1991. - STAMPA. - 144:6(2017), pp. 1035-1044. [10.1242/dev.142836]
File allegati a questo prodotto
File Dimensione Formato  
Pievani_Human-umbilical_2017.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 2.9 MB
Formato Adobe PDF
2.9 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/953760
Citazioni
  • ???jsp.display-item.citation.pmc??? 10
  • Scopus 22
  • ???jsp.display-item.citation.isi??? 18
social impact