The composition of skeletal muscle was investigated in the quadriceps and gastrocnemius muscle of 13-monthold (n=15) and 23-month-old (n=19) mice by means of high-resolution nuclear magnetic resonance (NMR) spectroscopy. Muscle specimens were dissected out, frozen in liquid nitrogen and extracted in chloroform/methanol, and proton NMR spectra of the resulting aqueous and organic fractions were obtained at 600 MHz. Several metabolites were unambiguously identified and quantified. Multivariate ANOVA (factor: age, muscle, age × muscle) showed a significant main effect of age (P=0.031) on the amount of muscle metabolites, suggesting that the aging process affects the composition of skeletal muscle. Univariate tests showed significant differences for lactate, acetate, taurine, and uridine in 13- and 23-month-old mice. A trend for the effect of muscle (quadriceps vs. gastrocnemius; P =0.128) was also found. No significant muscle x age interaction was present. When the same data were used in principal component analysis, the first two principal components separated muscles (quadriceps and gastrocnemius) and ages (13- and 23-month-old), explaining 66.7% of total variance. The results of this pilot study show that high-resolution NMR spectroscopy is able to detect age-associated changes in skeletal muscle metabolites, thereby paving the way to future detailed metabolomics investigation in sarcopenia of aging.
Age-related changes in skeletal muscle composition: a pilot nuclear magnetic resonance spectroscopy study in mice / Sobolev, Anatoly P.; Mannina, Luisa; Costanzo, Manuela; Cisterna, Barbara; Malatesta, Manuela; Zancanaro, Carlo. - In: EXPERIMENTAL GERONTOLOGY. - ISSN 0531-5565. - ELETTRONICO. - 92:(2017), pp. 23-27. [10.1016/j.exger.2017.03.005]
Age-related changes in skeletal muscle composition: a pilot nuclear magnetic resonance spectroscopy study in mice
MANNINA, LUISA;
2017
Abstract
The composition of skeletal muscle was investigated in the quadriceps and gastrocnemius muscle of 13-monthold (n=15) and 23-month-old (n=19) mice by means of high-resolution nuclear magnetic resonance (NMR) spectroscopy. Muscle specimens were dissected out, frozen in liquid nitrogen and extracted in chloroform/methanol, and proton NMR spectra of the resulting aqueous and organic fractions were obtained at 600 MHz. Several metabolites were unambiguously identified and quantified. Multivariate ANOVA (factor: age, muscle, age × muscle) showed a significant main effect of age (P=0.031) on the amount of muscle metabolites, suggesting that the aging process affects the composition of skeletal muscle. Univariate tests showed significant differences for lactate, acetate, taurine, and uridine in 13- and 23-month-old mice. A trend for the effect of muscle (quadriceps vs. gastrocnemius; P =0.128) was also found. No significant muscle x age interaction was present. When the same data were used in principal component analysis, the first two principal components separated muscles (quadriceps and gastrocnemius) and ages (13- and 23-month-old), explaining 66.7% of total variance. The results of this pilot study show that high-resolution NMR spectroscopy is able to detect age-associated changes in skeletal muscle metabolites, thereby paving the way to future detailed metabolomics investigation in sarcopenia of aging.File | Dimensione | Formato | |
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