An N-terminal polypeptide (XVAX2.P104) of the XVAX2 protein, which is involved in controlling the dorsoventral patterning of the retina in Xenopus laevis, was expressed and purified as a Histagged fusion protein (pHis-XVAX2-P104), and it was employed to generate an anti-XVAX2 antibody in New Zealand white rabbits. ELISA analysis shows that the titer of the antibody is as high as ∼1:100,000. The antibody could specifically recognize the full-length XVAX2 protein in extracts of Xenopus embryos, as determined by Western Blotting.

Expression of recombinant XVAX2 peptide-104 of Xenopus laevis and preparation of the antibody / Liu, Yang; Liu, Ying; Liu, Wei; Lupo, Giuseppe; He, Rong Qiao; Barsacchi, Giuseppina. - In: PROTEIN AND PEPTIDE LETTERS. - ISSN 0929-8665. - STAMPA. - 10:2(2003), pp. 213-219. [10.2174/0929866033479086]

Expression of recombinant XVAX2 peptide-104 of Xenopus laevis and preparation of the antibody

LUPO, GIUSEPPE;
2003

Abstract

An N-terminal polypeptide (XVAX2.P104) of the XVAX2 protein, which is involved in controlling the dorsoventral patterning of the retina in Xenopus laevis, was expressed and purified as a Histagged fusion protein (pHis-XVAX2-P104), and it was employed to generate an anti-XVAX2 antibody in New Zealand white rabbits. ELISA analysis shows that the titer of the antibody is as high as ∼1:100,000. The antibody could specifically recognize the full-length XVAX2 protein in extracts of Xenopus embryos, as determined by Western Blotting.
2003
Biochemistry; Molecular Biology
01 Pubblicazione su rivista::01a Articolo in rivista
Expression of recombinant XVAX2 peptide-104 of Xenopus laevis and preparation of the antibody / Liu, Yang; Liu, Ying; Liu, Wei; Lupo, Giuseppe; He, Rong Qiao; Barsacchi, Giuseppina. - In: PROTEIN AND PEPTIDE LETTERS. - ISSN 0929-8665. - STAMPA. - 10:2(2003), pp. 213-219. [10.2174/0929866033479086]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/950921
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