S-adenosylmethionine is a metabolite regulating many biological processes; S-adenosylmethionine effect on ubiquitin-proteasome system (UPS) has not been studied yet. We investigated S-adenosylmethionine effects on UPS activity both in vitro, by inhibitor screening assay, and in rat vascular smooth muscle cells, by Western Blot of proteasomal targets. We found that S-adenosylmethionine inhibited UPS activity.
S-adenosylmethionine inhibits ubiquitin-proteasome system in vitro and on rat vascular smooth muscle cells / Cucina, Alessandra; Cavallaro, Giuseppe; Bizzarri, Mariano; Cavallaro, Rosaria Adele; D'Anselmi, Fabrizio; Cavallaro, Antonino; Fuso, Andrea; Scarpa, Sigfrido. - In: PROTEIN AND PEPTIDE LETTERS. - ISSN 0929-8665. - STAMPA. - 15:1(2008), pp. 58-62. [10.2174/092986608783330396]
S-adenosylmethionine inhibits ubiquitin-proteasome system in vitro and on rat vascular smooth muscle cells
Cucina, Alessandra;Cavallaro, Giuseppe;BIZZARRI, Mariano;D'ANSELMI, FABRIZIO;CAVALLARO, Antonino;FUSO, ANDREA;SCARPA, Sigfrido
2008
Abstract
S-adenosylmethionine is a metabolite regulating many biological processes; S-adenosylmethionine effect on ubiquitin-proteasome system (UPS) has not been studied yet. We investigated S-adenosylmethionine effects on UPS activity both in vitro, by inhibitor screening assay, and in rat vascular smooth muscle cells, by Western Blot of proteasomal targets. We found that S-adenosylmethionine inhibited UPS activity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
