The viral surface glycoprotein neuraminidase (NA) allows the influenza virus penetration and the egress of virions. NAs are classified as A. B, and C. Type-A NAs from influenza virus are subdivided into two phylogenetically distinct families, group-1 and group-2. NA inhibition by oseltamivir represents a therapeutic approach against the avian influenza virus H5N1. Here, structural bases for oseltamivir recognition by group-1 NA1, NA8 and group-2 NA9 are highlighted by the ScrewFit algorithm for quantitative structure comparison. Oseltamivir binding to NA1 and NA8 affects the geometry of Glu119 and of regions Arg130-Ser160, Val240-Gly260, and Asp330-Glu382, leading to multiple NA conformations. Additionally, although NA1 and NA9 share almost the same oseltamivir-bound final conformation, they show some relevant differences as suggested by the ScrewFit algorithm. These results indicate that the design of new NA inhibitors should take into account these family-specific effects induced on the whole structure of NAs. (C) 2009 Elsevier B.V. All rights reserved.

Inhibition of viral group-1 and group-2 neuraminidases by oseltamivir: A comparative structural analysis by the ScrewFit algorithm / Paolo A., Calligari; Gerald R., Kneller; Giansanti, Andrea; Paolo, Ascenzi; Alessandro, Porrello; Alessio, Bocedi. - In: BIOPHYSICAL CHEMISTRY. - ISSN 0301-4622. - 141:1(2009), pp. 117-123. [10.1016/j.bpc.2009.01.004]

Inhibition of viral group-1 and group-2 neuraminidases by oseltamivir: A comparative structural analysis by the ScrewFit algorithm

GIANSANTI, Andrea;
2009

Abstract

The viral surface glycoprotein neuraminidase (NA) allows the influenza virus penetration and the egress of virions. NAs are classified as A. B, and C. Type-A NAs from influenza virus are subdivided into two phylogenetically distinct families, group-1 and group-2. NA inhibition by oseltamivir represents a therapeutic approach against the avian influenza virus H5N1. Here, structural bases for oseltamivir recognition by group-1 NA1, NA8 and group-2 NA9 are highlighted by the ScrewFit algorithm for quantitative structure comparison. Oseltamivir binding to NA1 and NA8 affects the geometry of Glu119 and of regions Arg130-Ser160, Val240-Gly260, and Asp330-Glu382, leading to multiple NA conformations. Additionally, although NA1 and NA9 share almost the same oseltamivir-bound final conformation, they show some relevant differences as suggested by the ScrewFit algorithm. These results indicate that the design of new NA inhibitors should take into account these family-specific effects induced on the whole structure of NAs. (C) 2009 Elsevier B.V. All rights reserved.
2009
comparative structural analysis; enzyme structure; neuraminidase; oseltamivir binding; screwfit algorithm
01 Pubblicazione su rivista::01a Articolo in rivista
Inhibition of viral group-1 and group-2 neuraminidases by oseltamivir: A comparative structural analysis by the ScrewFit algorithm / Paolo A., Calligari; Gerald R., Kneller; Giansanti, Andrea; Paolo, Ascenzi; Alessandro, Porrello; Alessio, Bocedi. - In: BIOPHYSICAL CHEMISTRY. - ISSN 0301-4622. - 141:1(2009), pp. 117-123. [10.1016/j.bpc.2009.01.004]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/94310
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