The thesis presents the design and the experimental development of a compact and high sensitive Lab-on-Chip (LoC) system suitable for the implementation of SELEX technique. SELEX (Systematic Evolution of Ligands by EXponential enrichment) is a combinatorial technique used in molecular biology to produce copies of the same nucleotide and to select a strand of DNA (aptamer) specific for a target molecule. The proposed Lab-On-Chip system includes the following functional units: an amplification module based on the PCR technique; a separation module able to obtain a single strand DNA from a double strand DNA and a selection module for the specific selection of the aptamer. These functionalities are implemented combining microfluidic components (micro-channels and micro-valves), electronic devices (amorphous silicon photosensors, thin film heaters, temperature sensors) and bioanalytical procedure.
Implementation of SELEX technique on Lab-on-Chip systems / Petrucci, Giulia. - (2017 Feb 27).
Implementation of SELEX technique on Lab-on-Chip systems
PETRUCCI, GIULIA
27/02/2017
Abstract
The thesis presents the design and the experimental development of a compact and high sensitive Lab-on-Chip (LoC) system suitable for the implementation of SELEX technique. SELEX (Systematic Evolution of Ligands by EXponential enrichment) is a combinatorial technique used in molecular biology to produce copies of the same nucleotide and to select a strand of DNA (aptamer) specific for a target molecule. The proposed Lab-On-Chip system includes the following functional units: an amplification module based on the PCR technique; a separation module able to obtain a single strand DNA from a double strand DNA and a selection module for the specific selection of the aptamer. These functionalities are implemented combining microfluidic components (micro-channels and micro-valves), electronic devices (amorphous silicon photosensors, thin film heaters, temperature sensors) and bioanalytical procedure.File | Dimensione | Formato | |
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Tesi dottorato Petrucci
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