A catalase-based (NAF/MWCNTs) nanocomposite film modified glassy carbon electrode for hydrogen peroxide (H2O2) detection was developed. The developed biosensor was characterized in terms of its bioelectrochemical properties. Cyclic voltammetry (CV) technique was employed to study the redox features of the enzyme in the absence and in the presence of nanomaterials dispersed in Nafion polymeric solution. The electron transfer coefficient, , and the electron transfer rate constant, , were found to be 0.42 and 1.71 s−1, at pH 7.0, respectively. Subsequently, the same modification steps were applied to mesoporous graphite screenprinted electrodes. Also, these electrodes were characterized in terms of their main electrochemical and kinetic parameters. The biosensor performances improved considerably after modification with nanomaterials. Moreover, the association of Nafion with carbon nanotubes retained the biological activity of the redox protein. The enzyme electrode response was linear in the range 2.5– 1150 mol L−1, with LOD of 0.83 mol L−1. From the experimental data, we can assess the possibility of using the modified biosensor as a useful tool for H2O2 determination in packaged beverages.

Catalase-based modified graphite electrode for hydrogen peroxide detection in different beverages / Fusco, Giovanni; Bollella, Paolo; Mazzei, Franco; Favero, Gabriele; Antiochia, Riccarda; Tortolini, Cristina. - In: JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY. - ISSN 2090-8873. - STAMPA. - 2016:(2016), pp. 1-12. [10.1155/2016/8174913]

Catalase-based modified graphite electrode for hydrogen peroxide detection in different beverages

FUSCO, GIOVANNI;BOLLELLA, PAOLO;MAZZEI, Franco;FAVERO, Gabriele;ANTIOCHIA, RICCARDA;TORTOLINI, CRISTINA
2016

Abstract

A catalase-based (NAF/MWCNTs) nanocomposite film modified glassy carbon electrode for hydrogen peroxide (H2O2) detection was developed. The developed biosensor was characterized in terms of its bioelectrochemical properties. Cyclic voltammetry (CV) technique was employed to study the redox features of the enzyme in the absence and in the presence of nanomaterials dispersed in Nafion polymeric solution. The electron transfer coefficient, , and the electron transfer rate constant, , were found to be 0.42 and 1.71 s−1, at pH 7.0, respectively. Subsequently, the same modification steps were applied to mesoporous graphite screenprinted electrodes. Also, these electrodes were characterized in terms of their main electrochemical and kinetic parameters. The biosensor performances improved considerably after modification with nanomaterials. Moreover, the association of Nafion with carbon nanotubes retained the biological activity of the redox protein. The enzyme electrode response was linear in the range 2.5– 1150 mol L−1, with LOD of 0.83 mol L−1. From the experimental data, we can assess the possibility of using the modified biosensor as a useful tool for H2O2 determination in packaged beverages.
2016
analytical chemistry; chemical engineering (all); instrumentation; computer science applications1707; computer vision and pattern recognition
01 Pubblicazione su rivista::01a Articolo in rivista
Catalase-based modified graphite electrode for hydrogen peroxide detection in different beverages / Fusco, Giovanni; Bollella, Paolo; Mazzei, Franco; Favero, Gabriele; Antiochia, Riccarda; Tortolini, Cristina. - In: JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY. - ISSN 2090-8873. - STAMPA. - 2016:(2016), pp. 1-12. [10.1155/2016/8174913]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/934121
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