Despite tea increased plasma nonenzymatic antioxidant capacity, the European Food Safety Administration (EFSA) denied claims related to tea and its protection from oxidative damage. Furthermore, the Supplement Information Expert Committee (DSI EC) expressed some doubts on the safety of green tea extract (GTE). We performed a pilot study in order to evaluate the effect of a single dose of two capsules of a GTE supplement (200mg x 2) on the peroxidation of leukocytes index ratio (PLIR) in relation to uric acid (UA) and ferric reducing antioxidant potential (FRAP), as well as the sample size to reach statistical significance. GTE induced a prooxidant effect on leukocytes, whereas FRAP did not change, in agreement with the EFSA and the DSI EC conclusions. Besides, our results confirm the primary role of UA in the antioxidant defences. The ratio based calculation of the PLIR reduced the sample size to reach statistical significance, compared to the resistance to an exogenous oxidative stress and to the functional capacity of oxidative burst. Therefore, PLIR could be a sensitive marker of redox status.

The peroxidation of leukocytes index ratio reveals the prooxidant effect of green tea extract / Peluso, Ilaria; Manafikhi, Husseen; Raguzzini, Anna; Longhitano, Yaroslava; Reggi, Raffaella; Zanza, Christian; Palmery, Maura. - In: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY. - ISSN 1942-0900. - ELETTRONICO. - 2016:(2016). [10.1155/2016/9139731]

The peroxidation of leukocytes index ratio reveals the prooxidant effect of green tea extract

PELUSO, Ilaria;MANAFIKHI, HUSSEEN;REGGI, RAFFAELLA;ZANZA, CHRISTIAN;PALMERY, Maura
2016

Abstract

Despite tea increased plasma nonenzymatic antioxidant capacity, the European Food Safety Administration (EFSA) denied claims related to tea and its protection from oxidative damage. Furthermore, the Supplement Information Expert Committee (DSI EC) expressed some doubts on the safety of green tea extract (GTE). We performed a pilot study in order to evaluate the effect of a single dose of two capsules of a GTE supplement (200mg x 2) on the peroxidation of leukocytes index ratio (PLIR) in relation to uric acid (UA) and ferric reducing antioxidant potential (FRAP), as well as the sample size to reach statistical significance. GTE induced a prooxidant effect on leukocytes, whereas FRAP did not change, in agreement with the EFSA and the DSI EC conclusions. Besides, our results confirm the primary role of UA in the antioxidant defences. The ratio based calculation of the PLIR reduced the sample size to reach statistical significance, compared to the resistance to an exogenous oxidative stress and to the functional capacity of oxidative burst. Therefore, PLIR could be a sensitive marker of redox status.
2016
biochemistry; aging; cell biology
01 Pubblicazione su rivista::01a Articolo in rivista
The peroxidation of leukocytes index ratio reveals the prooxidant effect of green tea extract / Peluso, Ilaria; Manafikhi, Husseen; Raguzzini, Anna; Longhitano, Yaroslava; Reggi, Raffaella; Zanza, Christian; Palmery, Maura. - In: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY. - ISSN 1942-0900. - ELETTRONICO. - 2016:(2016). [10.1155/2016/9139731]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/927320
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