The search for novel lipid A analogues from any biological source that can act as antagonists, displaying inhibitory activity towards the production of pro-inflammatory cytokines, or as immunomodulators in mammals, is a very topical issue. To this aim, the structure and immunological properties of the lipopolysaccharide lipid A from the purple nonsulfur bacterium Rhodopseudomonas palustris strain BisA53 have been determined. This lipid A displays a unique structural feature, with a non-phosphorylated skeleton made up of the tetrasaccharide Manp-α-(1→4)-GlcpN3N-β-1→6-GlcpN3N-α-(1→1)-α-GalpA, and four primary amide-linked 14:0(3-OH) and, as secondary O-acyl substituents, a 16:0 and the very long-chain fatty acid 26:0(25-OAc), appended on the GlcpN3N units. This lipid A architecture is definitely rare, so far identified only in the genus Bradyrhizobium. Immunological tests on both murine bone-marrow-derived and human monocyte-derived macrophages revealed an extremely low immunostimulant capability of this LPS lipid A.

he search for novel lipid A analogues from anybiological source that can act as antagonists, displaying in-hibitory activity towards the production of pro-inflammatorycytokines, or as immunomodulators in mammals, is a verytopical issue. To this aim, the structure and immunologicalproperties of the lipopolysaccharide lipid A from the purplenonsulfur bacterium Rhodopseudomonas palustris strainBisA53 have been determined. This lipid A displays a uniquestructural feature, with a non-phosphorylated skeleton madeup of the tetrasacchari de Manp-a-(1!4)-GlcpN3N-b-1!6-GlcpN3N-a-(1!1)-a-GalpA, and four primary amide-linked14:0(3-OH) and, as secondary O-acyl substituents, a 16:0 andthe very long-chain fatty acid 26:0(25-OAc), appended onthe GlcpN3N units. This lipid A architecture is definitely rare,so far identified only in the genus Bradyrhizobium. Immuno-logical tests on both murine bone-marro w-derived andhuman monocyte-derived macrophages revealed an ex-tremely low immunostimulant capability of this LPS lipid A.

The lipid a from Rhodopseudomonas palustris strain BiSa53 LPS possesses a unique structure and low immunostimulant properties / Dilorenzo, Flaviana; Palmigiano, Angelo; AL BITAR NEHME, Sami; Sturiale, Luisa; Duda, Katarzyna A.; Gully, Djamel; Lanzetta, Rosa; Giraud, Eric; Garozzo, Domenico; Bernardini, Maria; Molinaro, Antonio; Silipo, Alba. - In: CHEMISTRY-A EUROPEAN JOURNAL. - ISSN 0947-6539. - STAMPA. - 23:15(2017), pp. 3637-3647. [10.1002/chem.201604379]

The lipid a from Rhodopseudomonas palustris strain BiSa53 LPS possesses a unique structure and low immunostimulant properties

AL BITAR NEHME, SAMI;BERNARDINI, Maria;
2017

Abstract

The search for novel lipid A analogues from any biological source that can act as antagonists, displaying inhibitory activity towards the production of pro-inflammatory cytokines, or as immunomodulators in mammals, is a very topical issue. To this aim, the structure and immunological properties of the lipopolysaccharide lipid A from the purple nonsulfur bacterium Rhodopseudomonas palustris strain BisA53 have been determined. This lipid A displays a unique structural feature, with a non-phosphorylated skeleton made up of the tetrasaccharide Manp-α-(1→4)-GlcpN3N-β-1→6-GlcpN3N-α-(1→1)-α-GalpA, and four primary amide-linked 14:0(3-OH) and, as secondary O-acyl substituents, a 16:0 and the very long-chain fatty acid 26:0(25-OAc), appended on the GlcpN3N units. This lipid A architecture is definitely rare, so far identified only in the genus Bradyrhizobium. Immunological tests on both murine bone-marrow-derived and human monocyte-derived macrophages revealed an extremely low immunostimulant capability of this LPS lipid A.
2017
he search for novel lipid A analogues from anybiological source that can act as antagonists, displaying in-hibitory activity towards the production of pro-inflammatorycytokines, or as immunomodulators in mammals, is a verytopical issue. To this aim, the structure and immunologicalproperties of the lipopolysaccharide lipid A from the purplenonsulfur bacterium Rhodopseudomonas palustris strainBisA53 have been determined. This lipid A displays a uniquestructural feature, with a non-phosphorylated skeleton madeup of the tetrasacchari de Manp-a-(1!4)-GlcpN3N-b-1!6-GlcpN3N-a-(1!1)-a-GalpA, and four primary amide-linked14:0(3-OH) and, as secondary O-acyl substituents, a 16:0 andthe very long-chain fatty acid 26:0(25-OAc), appended onthe GlcpN3N units. This lipid A architecture is definitely rare,so far identified only in the genus Bradyrhizobium. Immuno-logical tests on both murine bone-marro w-derived andhuman monocyte-derived macrophages revealed an ex-tremely low immunostimulant capability of this LPS lipid A.
Fatty acids; Glycolipids; Innate immunity; Lipopolysaccharides; NMR spectroscopy; Chemistry (all)
01 Pubblicazione su rivista::01a Articolo in rivista
The lipid a from Rhodopseudomonas palustris strain BiSa53 LPS possesses a unique structure and low immunostimulant properties / Dilorenzo, Flaviana; Palmigiano, Angelo; AL BITAR NEHME, Sami; Sturiale, Luisa; Duda, Katarzyna A.; Gully, Djamel; Lanzetta, Rosa; Giraud, Eric; Garozzo, Domenico; Bernardini, Maria; Molinaro, Antonio; Silipo, Alba. - In: CHEMISTRY-A EUROPEAN JOURNAL. - ISSN 0947-6539. - STAMPA. - 23:15(2017), pp. 3637-3647. [10.1002/chem.201604379]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/926925
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