High-performance liquid chromatographic analysis of norfloxacin in human tissues and plasma with fluorescence detection

A high-performance liquid chromatographic analysis with fluorimetric detection is described for the quantitative determination of norfloxacin in renal, prostatic tissues and in plasma. The analytical procedure in the tissue pretreatment, consists of purification of the obtained sample by a solid state extraction and quantitation by HPLC. The samples were chromatographed on a C8 reversed-phase column. Analytical recoveries ranged from 95.2 to 97.6%. Within and between day precision were assessed by analysing serum containing 50 and 500 ng/ml norfloxacin. At each concentration, within day precision was ≤ 3.6% (relative standard deviation, n = 10) and day-to-day precision was ≤ 5.3% (n = 10). Limit of detection was ca 1 ng/ml.