The trimeric translation initiation factor a/eIF2 of the crenarchaeon Sulfolobus solfataricus is pivotal for binding of initiator tRNA to the ribosome. Here, we present in vitro and in vivo evidence that the a/eIF2 gamma-subunit exhibits an additional function with resemblance to the eukaryotic cap-complex. It binds to the 5'-triphosphate end of mRNA and protects the 5' part from degradation. This unprecedented capacity of the archaeal initiation factor further indicates that 5' --> 3' directional mRNA decay is a pathway common to all domains of life.
Translation initiation factor a/eIF2(-gamma) counteracts 5' to 3' mRNA decay in the archaeon Sulfolobus solfataricus / D., Hasenoehrl; T., Lombo; V., Kaberdin; Londei, Paola; U., Blaesi. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 1091-6490. - 105:6(2008), pp. 2146-2150. [10.1073/pnas.0708894105]
Translation initiation factor a/eIF2(-gamma) counteracts 5' to 3' mRNA decay in the archaeon Sulfolobus solfataricus.
LONDEI, Paola;
2008
Abstract
The trimeric translation initiation factor a/eIF2 of the crenarchaeon Sulfolobus solfataricus is pivotal for binding of initiator tRNA to the ribosome. Here, we present in vitro and in vivo evidence that the a/eIF2 gamma-subunit exhibits an additional function with resemblance to the eukaryotic cap-complex. It binds to the 5'-triphosphate end of mRNA and protects the 5' part from degradation. This unprecedented capacity of the archaeal initiation factor further indicates that 5' --> 3' directional mRNA decay is a pathway common to all domains of life.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
