Over the past decades, the effects of ultrashort-pulsed electric fields have been used to investigate their action in many medical applications (e.g. cancer, gene electrotransfer, drug delivery, electrofusion). Promising aspects of these pulses has led to several in vitro and in vivo experiments to clarify their action. Since the basic mechanisms of these pulses have not yet been fully clarified, scientific interest has focused on the development of numerical models at different levels of complexity: atomic (molecular dynamic simulations), microscopic (microdosimetry) and macroscopic (dosimetry). The aim of this work is to demonstrate that, in order to predict results at the cellular level, an accurate microdosimetry model is needed using a realistic cell shape, and with their position and packaging (cell density) characterised inside the medium.

A microdosimetric study of electropulsation on multiple realistically shaped cells. Effect of Neighbours / Denzi, Agnese; Camera, Francesca; Merla, Caterina; Benassi, Barbara; Consales, Claudia; Paffi, Alessandra; Apollonio, Francesca; Liberti, Micaela. - In: THE JOURNAL OF MEMBRANE BIOLOGY. - ISSN 0022-2631. - ELETTRONICO. - 249:5(2016), pp. 691-701. [10.1007/s00232-016-9912-3]

A microdosimetric study of electropulsation on multiple realistically shaped cells. Effect of Neighbours

Camera, Francesca;PAFFI, ALESSANDRA;APOLLONIO, Francesca;LIBERTI, Micaela
2016

Abstract

Over the past decades, the effects of ultrashort-pulsed electric fields have been used to investigate their action in many medical applications (e.g. cancer, gene electrotransfer, drug delivery, electrofusion). Promising aspects of these pulses has led to several in vitro and in vivo experiments to clarify their action. Since the basic mechanisms of these pulses have not yet been fully clarified, scientific interest has focused on the development of numerical models at different levels of complexity: atomic (molecular dynamic simulations), microscopic (microdosimetry) and macroscopic (dosimetry). The aim of this work is to demonstrate that, in order to predict results at the cellular level, an accurate microdosimetry model is needed using a realistic cell shape, and with their position and packaging (cell density) characterised inside the medium.
2016
Electroporation; mesodosimetry; microdosimetry; nanosecond and microsecond pulsed electric fields; shielding effect; biophysics; physiology; cell biology
01 Pubblicazione su rivista::01a Articolo in rivista
A microdosimetric study of electropulsation on multiple realistically shaped cells. Effect of Neighbours / Denzi, Agnese; Camera, Francesca; Merla, Caterina; Benassi, Barbara; Consales, Claudia; Paffi, Alessandra; Apollonio, Francesca; Liberti, Micaela. - In: THE JOURNAL OF MEMBRANE BIOLOGY. - ISSN 0022-2631. - ELETTRONICO. - 249:5(2016), pp. 691-701. [10.1007/s00232-016-9912-3]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/907339
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