Neuroblastoma is one of the most common paediatric solid tumors originating from the sympathoadrenal lineage of neural crest. This tumor shows extremely different clinical phenotypes such as spontaneous regression on one hand and aggressive growth on the other hand. Undifferentiated neuroblastoma cell line (Lan-5) represent a good model to study neuronal differentiation induced by a variety of stimuli such as retinoic acid treatment. At scanning electron microscopy analysis this cells showed rare neurite-like processes containing a small amount of neurosecretory granules and a low expression of the neurofilaments polypeptides. In our experiments the exposure for 5 days to 50 Hz, 2 mT magnetic field inhibits cell growth proliferation with a decrease of 30 % in exposed cells respect to the control. Moreover at scanning electron microscopy exposed cells showed a neuronal morphology aspect and development of a larger amount of neuritic like processes than in the control cells. Also the immunofluorescent analysis of 50 Hz, 2mT exposed cells shows an enhancement on the expression of NF200. These results suggest that exposition of Lan-5 to 50 Hz, 2mT magnetic field can interfere with cell growth process and induce a neuronal like cell morphology.

Effects of 50 Hz magnetic field exposure on Neuroblastoma morphology / Pozzi, Deleana; Grimaldi, S; Ledda, M; DE CARLO, F; Modesti, A; Scarpa, Susanna; Foletti, A. AND LISI A.. - In: INTERNATIONAL JOURNAL OF INTEGRATIVE MEDICINE. - ISSN 1522-6255. - ELETTRONICO. - 1:(2007), pp. 12-17.

Effects of 50 Hz magnetic field exposure on Neuroblastoma morphology

POZZI, Deleana;SCARPA, Susanna;
2007

Abstract

Neuroblastoma is one of the most common paediatric solid tumors originating from the sympathoadrenal lineage of neural crest. This tumor shows extremely different clinical phenotypes such as spontaneous regression on one hand and aggressive growth on the other hand. Undifferentiated neuroblastoma cell line (Lan-5) represent a good model to study neuronal differentiation induced by a variety of stimuli such as retinoic acid treatment. At scanning electron microscopy analysis this cells showed rare neurite-like processes containing a small amount of neurosecretory granules and a low expression of the neurofilaments polypeptides. In our experiments the exposure for 5 days to 50 Hz, 2 mT magnetic field inhibits cell growth proliferation with a decrease of 30 % in exposed cells respect to the control. Moreover at scanning electron microscopy exposed cells showed a neuronal morphology aspect and development of a larger amount of neuritic like processes than in the control cells. Also the immunofluorescent analysis of 50 Hz, 2mT exposed cells shows an enhancement on the expression of NF200. These results suggest that exposition of Lan-5 to 50 Hz, 2mT magnetic field can interfere with cell growth process and induce a neuronal like cell morphology.
2007
01 Pubblicazione su rivista::01a Articolo in rivista
Effects of 50 Hz magnetic field exposure on Neuroblastoma morphology / Pozzi, Deleana; Grimaldi, S; Ledda, M; DE CARLO, F; Modesti, A; Scarpa, Susanna; Foletti, A. AND LISI A.. - In: INTERNATIONAL JOURNAL OF INTEGRATIVE MEDICINE. - ISSN 1522-6255. - ELETTRONICO. - 1:(2007), pp. 12-17.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/90421
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