AIM: To compare analytical characteristics between two different methods, a automated chemiluminescent immunoassay (CLIA) versus a radioimmunometric assay (RIA), for the determination of carbohydrate antigen 19-9 (CA19-9). MATERIALS AND METHODS: One hundred and eighty-five blood samples from consecutively enrolled individuals (87 males and 98 females, aged 22-89 and 29-89 years, respectively) were evaluated. For both assays, a cut-off of 37 U/ml was used. RESULTS:Comparison between assays was analyzed using Passing-Bablok regression, which showed a high inter-assay correlation (r=0.926). The mean intra-assay coefficient of variation was 17.6% (range=6.1-50.7%) for the RIA (n=10 samples) and 5.2% (range=0.4-17.0%) for the CLIA assay (n=26 samples). CONCLUSION: This study showed that CLIA for CA19-9 has a good reliability on all samples analyzed and should be preferred to RIA with the aim of reducing costs, greater standardization and more harmonized results.

Evaluation of an Automated CLIA System for the Determination of Carbohydrate Antigen 19-9 / Anastasi, Emanuela; DI SEGNI, Chantal; Tudini, Silvestra; Gennarini, Giuseppina; Angeloni, Antonio. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - ELETTRONICO. - 36:10(2016), pp. 5281-5285. [10.21873/anticanres.11099]

Evaluation of an Automated CLIA System for the Determination of Carbohydrate Antigen 19-9

ANASTASI, Emanuela;DI SEGNI, CHANTAL;TUDINI, Silvestra
Methodology
;
GENNARINI, Giuseppina;ANGELONI, Antonio
2016

Abstract

AIM: To compare analytical characteristics between two different methods, a automated chemiluminescent immunoassay (CLIA) versus a radioimmunometric assay (RIA), for the determination of carbohydrate antigen 19-9 (CA19-9). MATERIALS AND METHODS: One hundred and eighty-five blood samples from consecutively enrolled individuals (87 males and 98 females, aged 22-89 and 29-89 years, respectively) were evaluated. For both assays, a cut-off of 37 U/ml was used. RESULTS:Comparison between assays was analyzed using Passing-Bablok regression, which showed a high inter-assay correlation (r=0.926). The mean intra-assay coefficient of variation was 17.6% (range=6.1-50.7%) for the RIA (n=10 samples) and 5.2% (range=0.4-17.0%) for the CLIA assay (n=26 samples). CONCLUSION: This study showed that CLIA for CA19-9 has a good reliability on all samples analyzed and should be preferred to RIA with the aim of reducing costs, greater standardization and more harmonized results.
2016
Tumor marker; automated chemiluminescent immunoassay (CLIA); carbohydrate antigen 19-9 (CA19-9); radioimmunometric assay (RIA)
01 Pubblicazione su rivista::01a Articolo in rivista
Evaluation of an Automated CLIA System for the Determination of Carbohydrate Antigen 19-9 / Anastasi, Emanuela; DI SEGNI, Chantal; Tudini, Silvestra; Gennarini, Giuseppina; Angeloni, Antonio. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - ELETTRONICO. - 36:10(2016), pp. 5281-5285. [10.21873/anticanres.11099]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/899877
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