The gel electrophoretic permutation assays of DNA fragments experimentally investigated by different authors were theoretically reproduced using our theoretical model of sequence-dependent curvature. The general pattern of agreement obtained suggests that our method can be usefully adopted as an alternative to the experimental assay, in particular where the lack of a sufficient number of unique restriction sites in the fragment prevents the correct localization of the main bend site.
A theoretical method to predict DNA permutation gel electrophoresis from the sequence / D., Boffelli; DE SANTIS, Pasquale; Palleschi, Antonio; Risuleo, Gianfranco; Savino, Maria. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 300:(1992), pp. 175-178. [10.1016/0014-5793(92)80190-R]
A theoretical method to predict DNA permutation gel electrophoresis from the sequence.
DE SANTIS, Pasquale;PALLESCHI, Antonio;RISULEO, Gianfranco;SAVINO, Maria
1992
Abstract
The gel electrophoretic permutation assays of DNA fragments experimentally investigated by different authors were theoretically reproduced using our theoretical model of sequence-dependent curvature. The general pattern of agreement obtained suggests that our method can be usefully adopted as an alternative to the experimental assay, in particular where the lack of a sufficient number of unique restriction sites in the fragment prevents the correct localization of the main bend site.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.