The synthetic protein (Leu67-Lys33)100-Orn20 bound irreversibly to pBR322 circular DNA and induced supercoiling in the DNA. The no. of topoisomers reached an optimum corresponding to a wt.-wt. ratio of 1, then remained approx. const. The presence of a population of different topoisomers probably indicates nonregularity in the topol. effects caused by the nonuniformity of protein compn. and structure. A comparison between the effects on supercoiled and relaxed DNA shows that the protein binds most easily to supercoiled DNA. Two-dimensional electrophoresis in the presence of ethidium bromide indicates that the protein induces a left-handed supercoiling in DNA.
DNA supercoiling induced by a synthetic polypeptide / DE SANTIS, Pasquale; M., Falcioni; Morosetti, Stefano; Savino, Maria. - In: BIOPOLYMERS. - ISSN 0006-3525. - STAMPA. - 22:12(1983), pp. 2517-2521. [10.1002/bip.360221206]
DNA supercoiling induced by a synthetic polypeptide.
DE SANTIS, Pasquale;MOROSETTI, Stefano;SAVINO, Maria
1983
Abstract
The synthetic protein (Leu67-Lys33)100-Orn20 bound irreversibly to pBR322 circular DNA and induced supercoiling in the DNA. The no. of topoisomers reached an optimum corresponding to a wt.-wt. ratio of 1, then remained approx. const. The presence of a population of different topoisomers probably indicates nonregularity in the topol. effects caused by the nonuniformity of protein compn. and structure. A comparison between the effects on supercoiled and relaxed DNA shows that the protein binds most easily to supercoiled DNA. Two-dimensional electrophoresis in the presence of ethidium bromide indicates that the protein induces a left-handed supercoiling in DNA.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
