In this paper, multitasking core-shell Fe3O4@Cu@Au superparamagnetic nanoparticles (MNPs) functionalised either with folic acid (FA) or methotrexate (MTX) have been used for engulfment of human granulocytes. MNPs internalisation into phagocytes allows to use the cells as carriers thus avoiding the rapid clearance and decreasing of concentration commonly experienced in case of in vivo nanoparticles administration. MNPs have been coated with poly-L-lysine (PLL), which contributes to improve their dispersion into polar solvents. Moreover, this coating improves the cellular uptake and allows the further functionalisation with MTX and FA. Experimental evidences of granulocytes engulfment have been obtained by both SEM and fluorescence microscopy, upon prior labelling of the lysines residues with suitable fluorescent probes. The superparamagnetic nature of the MNPs makes the system easily drivable into a specific tissue upon exposure to a defined magnetic field. The success of the internalisation experiments indicates that the system is a promising tool as theranostic agent for treatment of a variety of diseases, including tumours.

Internalisation of core-shell superparamagnetic nanoparticles into human granulocytes / DE ANGELIS, Francesca; Berardi, Ginevra; Scaramuzzo, FRANCESCA ANNA; Liberatore, Mauro; Barteri, Mario. - In: INTERNATIONAL JOURNAL OF NANOTECHNOLOGY. - ISSN 1475-7435. - STAMPA. - 13:8-9(2016), pp. 659-666. [10.1504/IJNT.2016.079669]

Internalisation of core-shell superparamagnetic nanoparticles into human granulocytes

DE ANGELIS, Francesca;BERARDI, GINEVRA;SCARAMUZZO, FRANCESCA ANNA;LIBERATORE, Mauro;BARTERI, Mario
2016

Abstract

In this paper, multitasking core-shell Fe3O4@Cu@Au superparamagnetic nanoparticles (MNPs) functionalised either with folic acid (FA) or methotrexate (MTX) have been used for engulfment of human granulocytes. MNPs internalisation into phagocytes allows to use the cells as carriers thus avoiding the rapid clearance and decreasing of concentration commonly experienced in case of in vivo nanoparticles administration. MNPs have been coated with poly-L-lysine (PLL), which contributes to improve their dispersion into polar solvents. Moreover, this coating improves the cellular uptake and allows the further functionalisation with MTX and FA. Experimental evidences of granulocytes engulfment have been obtained by both SEM and fluorescence microscopy, upon prior labelling of the lysines residues with suitable fluorescent probes. The superparamagnetic nature of the MNPs makes the system easily drivable into a specific tissue upon exposure to a defined magnetic field. The success of the internalisation experiments indicates that the system is a promising tool as theranostic agent for treatment of a variety of diseases, including tumours.
2016
core-shell magnetic nanoparticles; folic acid; granulocytes; methotrexate; nanobiotechnology; bioengineering; condensed matter physics; materials chemistry2506 metals and alloys; electrical and electronic engineering
01 Pubblicazione su rivista::01a Articolo in rivista
Internalisation of core-shell superparamagnetic nanoparticles into human granulocytes / DE ANGELIS, Francesca; Berardi, Ginevra; Scaramuzzo, FRANCESCA ANNA; Liberatore, Mauro; Barteri, Mario. - In: INTERNATIONAL JOURNAL OF NANOTECHNOLOGY. - ISSN 1475-7435. - STAMPA. - 13:8-9(2016), pp. 659-666. [10.1504/IJNT.2016.079669]
File allegati a questo prodotto
File Dimensione Formato  
De Angelis_Internalisation_2016.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 2.17 MB
Formato Adobe PDF
2.17 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/894998
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 1
social impact