Genotyping wild and captive capuchins has become a priority and hair bulbs have high quality DNA. Here, we describe a method to non-invasively collect fresh-plucked strands of hair that exploits capuchins’ manual dexterity and propensity to grasp and extract food. The apparatus consists of a transparent tube baited with food. Its extraction requires the monkey to place its forearm in contact with double-sided tape applied on the inner surface of the tube entrance. The “tube” method, successfully implemented with captive (N=23) and wild (N=21) capuchins, allowed us to obtain hair bulbs from most individuals and usable genomic DNA was extracted even from a single bulb.
Making use of Capuchins’ behavioral propensities to obtain hair samples for DNA analyses / Visalberghi, Elisabetta; Cavallero, Serena; Fragaszy, Dorothy M.; Izar, Patricia; Aguiar, Rodrigo Mendes; Truppa, Valentina. - In: NEOTROPICAL PRIMATES. - ISSN 1413-4705. - ELETTRONICO. - 22:2(2015), pp. 89-93.
Making use of Capuchins’ behavioral propensities to obtain hair samples for DNA analyses
CAVALLERO, Serena;
2015
Abstract
Genotyping wild and captive capuchins has become a priority and hair bulbs have high quality DNA. Here, we describe a method to non-invasively collect fresh-plucked strands of hair that exploits capuchins’ manual dexterity and propensity to grasp and extract food. The apparatus consists of a transparent tube baited with food. Its extraction requires the monkey to place its forearm in contact with double-sided tape applied on the inner surface of the tube entrance. The “tube” method, successfully implemented with captive (N=23) and wild (N=21) capuchins, allowed us to obtain hair bulbs from most individuals and usable genomic DNA was extracted even from a single bulb.File | Dimensione | Formato | |
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