Comparison between bioluminescence imaging technique and CFU count for the study of oropharyngeal candidiasis in mice

We recently described a bioluminescence in vivo imaging technique, representing apowerful tool to test the real-time progression of oropharyngeal candidiasis, hencepotentially useful to evaluate the efficacy of antifungal therapies. In this study, the invivo imaging technique was compared with CFU measurement of target organs(tongue, esophagus and stomach) for monitoring and quantifying oropharyngeal can-didiasis. We have correlated these two analytical methods at different times post-infection using engineered, luminescent Candida albicans in mice rendered susceptibleto oral candidiasis by cortisone-acetate. Scatter plots, Pearson correlation and Student’st test were used to compare the methods. We observed that the bioluminescence in vivoimaging technique was more reliable than CFU counts in detecting early infection of,and its extent in, the oral cavity of the mouse. This was also evident following the intro-duction of a variable such as treatment with fluconazole. The results described in thisstudy could validate the bioluminescence in vivo imaging technique as a method tomonitor and quantify oropharyngeal candidiasis and to assess early discovery of activecompounds in vivo.