The preparation and structural organisation of new bioinspired nanomaterials based on regular alternating enantiomeric sequence of tetra- and hexapeptides end-linked to poly(ethylene glycol) (PEG) is reported. The peptide moiety is composed of two or three repeats of l-Ala-d-Val units while the PEG has a molecular weight of 2 kDa. The self-assembling properties of the two conjugates depend significantly on the length of the peptide. Nanoparticles with different sizes and morphologies are formed, the structural properties of which are compared with the previously studied l-Ala-d-Val octapeptide conjugate that self-assembles into rod-like nanoparticles. The aggregation properties were studied by NMR, circular dichroism, fluorescence spectroscopies and dynamic light scattering. The morphology and size of the nanoparticles were assessed by scanning electron microscopy and dynamic light scattering. The loading and release of a model drug were also investigated. This study demonstrates that, by changing the length of the peptide, it is possible to modulate the self-assembly and loading properties of peptide-PEG conjugates.

Self-assembly and drug release study of linear l,d-oligopeptide-poly(ethylene glycol) conjugates / Novelli, Federica; DE SANTIS, Serena; Punzi, Pasqualina; Giordano, CESARE GIOVANNI; Scipioni, Anita; Masci, Giancarlo. - In: NEW BIOTECHNOLOGY. - ISSN 1871-6784. - STAMPA. - 37:(2017), pp. 99-107. [10.1016/j.nbt.2016.07.005]

Self-assembly and drug release study of linear l,d-oligopeptide-poly(ethylene glycol) conjugates

DE SANTIS, Serena;SCIPIONI, Anita;MASCI, Giancarlo
2017

Abstract

The preparation and structural organisation of new bioinspired nanomaterials based on regular alternating enantiomeric sequence of tetra- and hexapeptides end-linked to poly(ethylene glycol) (PEG) is reported. The peptide moiety is composed of two or three repeats of l-Ala-d-Val units while the PEG has a molecular weight of 2 kDa. The self-assembling properties of the two conjugates depend significantly on the length of the peptide. Nanoparticles with different sizes and morphologies are formed, the structural properties of which are compared with the previously studied l-Ala-d-Val octapeptide conjugate that self-assembles into rod-like nanoparticles. The aggregation properties were studied by NMR, circular dichroism, fluorescence spectroscopies and dynamic light scattering. The morphology and size of the nanoparticles were assessed by scanning electron microscopy and dynamic light scattering. The loading and release of a model drug were also investigated. This study demonstrates that, by changing the length of the peptide, it is possible to modulate the self-assembly and loading properties of peptide-PEG conjugates.
2017
linear l,d-peptide; poly(ethylene glycol); conjugated l,d-peptide-polymer; self-assembly; nanoparticles; drug loading; sustained release
01 Pubblicazione su rivista::01a Articolo in rivista
Self-assembly and drug release study of linear l,d-oligopeptide-poly(ethylene glycol) conjugates / Novelli, Federica; DE SANTIS, Serena; Punzi, Pasqualina; Giordano, CESARE GIOVANNI; Scipioni, Anita; Masci, Giancarlo. - In: NEW BIOTECHNOLOGY. - ISSN 1871-6784. - STAMPA. - 37:(2017), pp. 99-107. [10.1016/j.nbt.2016.07.005]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/890065
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